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  • Featured Product
  • KD/KO Validated

ALKBH5 Recombinant antibody

ALKBH5 Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2K9

Cat no : 82083-1-RR

Synonyms

RNA demethylase ALKBH5, EC:1.14.11.53, Alpha-ketoglutarate-dependent dioxygenase alkB homolog 5, ALKBH 5, 2K9

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using ALKBH5 Recombinant antibody (82083-1-RR)

    WB analysis of HeLa using 82083-1-RR

    WB result of ALKBH5 antibody (82083-1-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALKBH5 transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using ALKBH5 Recombinant antibody (82083-1-RR)

    WB analysis using 82083-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82083-1-RR (ALKBH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using ALKBH5 Recombinant antibody (82083-1-RR)

    IF Staining of U-251 using 82083-1-RR

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using ALKBH5 antibody (82083-1-RR, Clone: 2K9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Affinity and Kinetic Characterization of 82083-1-RR

    Affinity and Kinetic Characterization of 82083-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82083-1-RR against Human ALKBH5 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, A549 cells, K-562 cells, mouse brain tissue, rat brain tissue
Positive IF/ICC detected inU-251 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82083-1-RR targets ALKBH5 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ALKBH5 fusion protein Ag10516 相同性解析による交差性が予測される生物種
Full Name alkB, alkylation repair homolog 5 (E. coli)
Calculated molecular weight 458 aa, 51 kDa
Observed molecular weight 40-50 kDa
GenBank accession numberBC062339
Gene symbol ALKBH5
Gene ID (NCBI) 54890
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ALKBH5, also named as ABH5 and OFOXD1, is a Dioxygenase that demethylates RNA by oxidative demethylation. It is a mammalian demethylase that oxidatively reverses m6A in mRNA in vitro and in vivo. ALKBH5 is required for spermatogenesis. There're some isoforms of ALKBH5 with MW 40-44 kDa and 52 kDa.

Protocols

Product Specific Protocols
WB protocol for ALKBH5 antibody 82083-1-RRDownload protocol
IF protocol for ALKBH5 antibody 82083-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols