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Host
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

5 Lipoxygenase Recombinant antibody, PBS Only

5 Lipoxygenase Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240740A10

Cat no : 83794-4-PBS

Synonyms

ALOX5, 5-LO, 5LO, 5-lipoxygenase, 5 LOX

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using 5 Lipoxygenase Recombinant antibody (83794-4-RR)

    WB analysis using 83794-4-RR (same clone as 83794-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83794-4-RR (ALOX5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83794-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovarian cancer using 5 Lipoxygenase Recombinant antibody (83794-4-RR)

    IHC staining of human ovarian cancer using 83794-4-RR (same clone as 83794-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83794-4-RR (5 Lipoxygenase antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83794-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83794-4-RR

    Affinity and Kinetic Characterization of 83794-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83794-4-RR against Human 5 Lipoxygenase were performed. The affinity constant is 0.392 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83794-4-PBS is 5 Lipoxygenase Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen 5 Lipoxygenase fusion protein Ag19503 相同性解析による交差性が予測される生物種
Full Name arachidonate 5-lipoxygenase
Calculated molecular weight 674 aa, 78 kDa
Observed molecular weight 70-78 kDa
GenBank accession numberBC130332
Gene symbol 5 Lipoxygenase
Gene ID (NCBI) 240
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ALOX5(arachidonate 5-lipoxygenase) is also named as LOG5, 5-LO and belongs to the lipoxygenase family. It catalyzes the first step in leukotriene biosynthesis, and plays a role in inflammatory processes. It is a 78 kDa iron-containing enzyme that translocates from the cytosol to associate with FLAP, and converts arachidonate in two steps into the unstable intermediate LTA4. It is essential for cysteinyl-leukotriene (cys-LT) production, critical mediators in asthma(PMID:12911785).