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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ALPL Polyclonal antibody, PBS Only

ALPL Polyclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

Cat no : 11187-1-PBS

Synonyms

Alkaline phosphatase, AKP2, AP, Alkaline phosphatase, tissue-nonspecific isozyme, Alkaline phosphatase tissue nonspecific isozyme

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ALPL Polyclonal antibody (11187-1-AP)

    WB analysis using 11187-1-AP (same clone as 11187-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 11187-1-AP (ALPL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11187-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ALPL Polyclonal antibody (11187-1-AP)

    IHC staining of human colon cancer using 11187-1-AP (same clone as 11187-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11187-1-AP (ALPL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11187-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using ALPL Polyclonal antibody (11187-1-AP)

    IHC staining of mouse kidney using 11187-1-AP (same clone as 11187-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11187-1-AP (ALPL antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11187-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ALPL Polyclonal antibody (11187-1-AP)

    IHC staining of human liver cancer using 11187-1-AP (same clone as 11187-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11187-1-AP (ALPL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11187-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using ALPL Polyclonal antibody (11187-1-AP)

    IF Staining of HEK-293 using 11187-1-AP (same clone as 11187-1-PBS)

    Immunofluorescent analysis of HEK-293 cells using 11187-1-AP (ALPL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11187-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using ALPL Polyclonal antibody (11187-1-AP)

    FC experiment of HeLa using 11187-1-AP (same clone as 11187-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human ALPL (11187-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 11187-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11187-1-PBS targets ALPL in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ALPL fusion protein Ag1664 相同性解析による交差性が予測される生物種
Full Name alkaline phosphatase, liver/bone/kidney
Calculated molecular weight 57 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC021289
Gene symbol ALPL
Gene ID (NCBI) 249
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. ALPL(Alkaline phosphatase, tissue-nonspecific isozyme) is also named as AP-TNAP, TNSALP and belongs to the alkaline phosphatase family. It can exsit as a homodimer with the molecular weight of 140 kDa(PMID:18724009). ALPL was synthesized as a 66-kDa endo-b-N-acetylglucos-aminidase H (Endo H)-sensitive form, and processed to an 80-kDa mature form, which is anchored to the plasma membrane via glycosylphosphatidylinositol (GPI)(PMID:10839996).This antibody can bind the four mentioned alkaline phosphatases.