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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ALPL Recombinant antibody

ALPL Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230212D5

Cat no : 82915-2-RR

Synonyms

Alkaline phosphatase, Alkaline phosphatase, tissue-nonspecific isozyme, Alkaline phosphatase tissue nonspecific isozyme, Alkaline phosphatase liver/bone/kidney isozyme, AKP2

Validation Data Gallery

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  • Western Blot (WB) analysis of L02 cells using ALPL Recombinant antibody (82915-2-RR)

    WB analysis of L02 using 82915-2-RR

    L02 cells were subjected to SDS PAGE followed by western blot with 82915-2-RR (ALPL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HeLa cells using ALPL Recombinant antibody (82915-2-RR)

    FC experiment of HeLa using 82915-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug ALPL Recombinant antibody (82915-2-RR, Clone:230212D5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inL02 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 82915-2-RR is ALPL Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ALPL fusion protein Ag1664 相同性解析による交差性が予測される生物種
Full Name alkaline phosphatase, liver/bone/kidney
Calculated molecular weight 57 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC021289
Gene symbol ALPL
Gene ID (NCBI) 249
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ALPL antibody 82915-2-RRDownload protocol
FC protocol for ALPL antibody 82915-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols