Validation Data Gallery
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Tested Applications
| Positive WB detected in | human placenta tissue, mouse liver tissue, HepG2 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 9 publications below |
| IHC | See 3 publications below |
Product Information
18507-1-AP targets PLAP in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PLAP fusion protein Ag13361 相同性解析による交差性が予測される生物種 |
| Full Name | alkaline phosphatase, placental (Regan isozyme) |
| Calculated molecular weight | 58 kDa |
| Observed molecular weight | 66 kDa |
| GenBank accession number | BC009647 |
| Gene Symbol | PLAP |
| Gene ID (NCBI) | 250 |
| RRID | AB_10644334 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P05187 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Placental alkaline phosphatase (PLAP) is a glycosyl phosphatidylinositol (GPI)-anchored sialoglycoprotein , which shows greater resistance to heat inactivation than other alkaline phosphatase. Presented at high levels in placental trophoblasts, PLAP is recognized as an important marker of differentiation in human malignancies.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PLAP antibody 18507-1-AP | Download protocol |
| IHC protocol for PLAP antibody 18507-1-AP | Download protocol |
| IP protocol for PLAP antibody 18507-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Nanobiotechnology Ultrasound-triggered piezoelectric polyetheretherketone with boosted osteogenesis via regulating Akt/GSK3β/β-catenin pathway | ||
Aging (Albany NY) Primary cilia regulate gastric cancer-induced bone loss via cilia/Wnt/β-catenin signaling pathway. | ||
ACS Biomater Sci Eng 6-Bromoindirubin-3'-oxime Promotes Osteogenic Differentiation of Periodontal Ligament Stem Cells and Facilitates Bone Regeneration in a Mouse Periodontitis Model. | ||
Mol Cell Endocrinol Long noncoding RNA ZFAS1 suppresses osteogenic differentiation of bone marrow-derived mesenchymal stem cells by upregulating miR-499-EPHA5 axis. | ||
Amino Acids New aspects of antiproliferative activity of 4-hydroxybenzyl isothiocyanate, a natural H2S-donor. |