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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ALPPL2 Recombinant antibody

ALPPL2 Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241867A12

Cat no : 84813-1-RR

Synonyms

ALPG, ALP-1, ALP1, Alkaline phosphatase, placental-like, Alkaline phosphatase, germ cell type

Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using ALPPL2 Recombinant antibody (84813-1-RR)

    WB analysis of human placenta using 84813-1-RR

    human placenta tissue were subjected to SDS PAGE followed by western blot with 84813-1-RR (ALPPL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using ALPPL2 Recombinant antibody (84813-1-RR)

    IHC staining of human placenta using 84813-1-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84813-1-RR (ALPPL2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using ALPPL2 Recombinant antibody (84813-1-RR)

    IHC staining of human placenta using 84813-1-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84813-1-RR (ALPPL2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inhuman placenta tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84813-1-RR is ALPPL2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ALPPL2 fusion protein Ag13360 相同性解析による交差性が予測される生物種
Full Name alkaline phosphatase, placental-like 2
Calculated molecular weight 57 kDa
Observed molecular weight70 kDa
GenBank accession numberBC014139
Gene symbol ALPPL2
Gene ID (NCBI) 251
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ALPPL2 (alkaline phosphatase placental-like 2), a member of the human alkaline phosphatase family, could be a potential biomarker for early diagnosis of Pancreatic ductal adenocarcinoma.

Protocols

Product Specific Protocols
WB protocol for ALPPL2 antibody 84813-1-RRDownload protocol
IHC protocol for ALPPL2 antibody 84813-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols