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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ALS2 Polyclonal antibody

ALS2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 13998-1-AP

Synonyms

PLSJ, IAHSP, ALSJ, Alsin, ALS2CR6

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ALS2 Polyclonal antibody (13998-1-AP)

    WB analysis using 13998-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13998-1-AP (ALS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using ALS2 Polyclonal antibody (13998-1-AP)

    WB analysis of HEK-293 using 13998-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 13998-1-AP (ALS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using ALS2 Polyclonal antibody (13998-1-AP)

    IP experiment of HEK-293 using 13998-1-AP

    IP result of anti-ALS2 (IP:13998-1-AP, 4ug; Detection:13998-1-AP 1:300) with HEK-293 cells lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using ALS2 Polyclonal antibody (13998-1-AP)

    IHC staining of human pancreas using 13998-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 13998-1-AP (ALS2 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using ALS2 Polyclonal antibody (13998-1-AP)

    IHC staining of human pancreas using 13998-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 13998-1-AP (ALS2 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using ALS2 Polyclonal antibody (13998-1-AP)

    IF Staining of HEK-293 using 13998-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 13998-1-AP (ALS2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse cerebellum tissue, HEK-293 cells, rat cerebellum tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13998-1-AP targets ALS2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ALS2 fusion protein Ag5087 相同性解析による交差性が予測される生物種
Full Name amyotrophic lateral sclerosis 2 (juvenile)
Calculated molecular weight 1657 aa, 184 kDa
Observed molecular weight 185 kDa
GenBank accession numberBC029174
Gene symbol ALS2
Gene ID (NCBI) 57679
RRIDAB_10858476
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ALS2 antibody 13998-1-APDownload protocol
IHC protocol for ALS2 antibody 13998-1-APDownload protocol
IF protocol for ALS2 antibody 13998-1-APDownload protocol
IP protocol for ALS2 antibody 13998-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols