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ALY Polyclonal antibody
ALY Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF/ICC, RIP, ELISA
Conjugate
Unconjugated
Cat no : 16690-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, HEK-293 cells, A431 cells |
Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:300-1:1200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 2 publications below |
WB | See 8 publications below |
IF | See 1 publications below |
RIP | See 1 publications below |
Product Information
16690-1-AP targets ALY in WB, IHC, IF/ICC, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, pig |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | ALY fusion protein Ag10044 相同性解析による交差性が予測される生物種 |
Full Name | THO complex 4 |
Calculated molecular weight | 257 aa, 27 kDa |
Observed molecular weight | 27-30 kDa |
GenBank accession number | BC052302 |
Gene symbol | ALY |
Gene ID (NCBI) | 10189 |
RRID | AB_2878300 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for ALY antibody 16690-1-AP | Download protocol |
IHC protocol for ALY antibody 16690-1-AP | Download protocol |
IF protocol for ALY antibody 16690-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Metab NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis. | ||
ACS Infect Dis Porcine ZC3H11A Is Essential for the Proliferation of Pseudorabies Virus and Porcine Circovirus 2.
| ||
PLoS Pathog TREX (transcription/export)-NP complex exerts a dual effect on regulating polymerase activity and replication of influenza A virus
| ||
Cell Death Dis Epigenetic addition of m5C to HBV transcripts promotes viral replication and evasion of innate antiviral responses | ||
Phytomedicine Niujiao Dihuang Jiedu decoction promotes SLC7A11 m5C methylation modification against ferroptosis in acute-on-chronic liver failure |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Roy (Verified Customer) (06-15-2024) | Weak band on WB from HeLa cells extracts but specific for ALYREF/THOC4 (1/1000 dilution, Overnight incubation at 4°C)
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