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KD/KO Validated

AMBRA1 Polyclonal antibody

Name: AMBRA1 Polyclonal antibody
Brand: Proteintech
SKU: 13762-1-AP

AMBRA1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 13762-1-AP

Synonyms

WDR94, KIAA1736, DDB1- and CUL4-associated factor 3, DCAF3, Activating molecule in BECN1-regulated autophagy protein 1

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis of MCF-7 using 13762-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 13762-1-AP (AMBRA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 13762-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 13762-1-AP (AMBRA1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HT-1080 using 13762-1-AP
HT-1080 cells were subjected to SDS PAGE followed by western blot with 13762-1-AP (AMBRA1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 13762-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 13762-1-AP (AMBRA1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 13762-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 13762-1-AP (AMBRA1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 13762-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 13762-1-AP (AMBRA1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human liver cancer using 13762-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13762-1-AP (AMBRA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 13762-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13762-1-AP (AMBRA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 13762-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using AMBRA1 antibody (13762-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
IF Staining of MCF-7 using 13762-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using AMBRA1 antibody (13762-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Cat No. 13762-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	MCF-7 cells, human brain tissue, HT-1080 cells, HepG2 cells, mouse brain tissue
Positive IHC detected in	human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells, MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 5 publications below
WB	See 14 publications below
IHC	See 4 publications below
IF	See 1 publications below
IP	See 2 publications below

Product Information

13762-1-AP targets AMBRA1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	AMBRA1 fusion protein Ag4735 相同性解析による交差性が予測される生物種
Full Name	autophagy/beclin-1 regulator 1
Calculated molecular weight	1208 aa, 133 kDa
Observed molecular weight	130-135 kDa
GenBank accession number	BC045609
Gene symbol	AMBRA1
Gene ID (NCBI)	55626
RRID	AB_10642007
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

AMBRA1 (Activating molecule in BECN1-regulated autophagy protein 1), also known as WDR94 or KIAA1736, is a 1,298 amino acid protein that contains three WD repeats. AMBRA1 regulates autophagy and development of the nervous system. Involved in autophagy in controlling protein turnover during neuronal development, and in regulating normal cell survival and proliferation. This antibody works well in WB, IHC and IF application.

Protocols

Product Specific Protocols
WB protocol for AMBRA1 antibody 13762-1-AP	Download protocol
IHC protocol for AMBRA1 antibody 13762-1-AP	Download protocol
IF protocol for AMBRA1 antibody 13762-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IHC	Nature CRL4AMBRA1 is a master regulator of D-type cyclins. Authors - Daniele Simoneschi KO Validated View Article
human	WB	Pharmacol Res miR-103a-3p regulates mitophagy in Parkinson's disease through Parkin/Ambra1 signaling. Authors - Junjun Zhou View Article
mouse	WB	Neurobiol Dis Neuron-astrocyte transmitophagy is altered in Alzheimer's disease. Authors - Riikka Lampinen View Article
human	WB,IHC	Brain Pathol AMBRA1, a novel α-synuclein-binding protein, is implicated in the pathogenesis of multiple system atrophy. Authors - Yasuo Miki KD Validated View Article
human	WB	Front Cell Dev Biol Autophagy-Related Genes and Long Noncoding RNAs Signatures as Predictive Biomarkers for Osteosarcoma Survival. Authors - Jian Zhang View Article
human	WB	Int J Biochem Cell Biol Selenoprotein S attenuates high glucose and/or ox-LDL-induced endothelium injury by regulating Akt/mTOR signaling and autophagy. Authors - Zinan Wang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AMBRA1 Polyclonal antibody

AMBRA1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis of MCF-7 using 13762-1-AP

WB analysis of MCF-7 using 13762-1-AP

WB analysis of HT-1080 using 13762-1-AP

WB analysis of HepG2 using 13762-1-AP

WB analysis of mouse brain using 13762-1-AP

WB analysis of human brain using 13762-1-AP

IHC staining of human liver cancer using 13762-1-AP

IHC staining of human liver cancer using 13762-1-AP

IF Staining of HepG2 using 13762-1-AP

IF Staining of MCF-7 using 13762-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

CRL4AMBRA1 is a master regulator of D-type cyclins.

miR-103a-3p regulates mitophagy in Parkinson's disease through Parkin/Ambra1 signaling.

Neuron-astrocyte transmitophagy is altered in Alzheimer's disease.

AMBRA1, a novel α-synuclein-binding protein, is implicated in the pathogenesis of multiple system atrophy.

Autophagy-Related Genes and Long Noncoding RNAs Signatures as Predictive Biomarkers for Osteosarcoma Survival.

Selenoprotein S attenuates high glucose and/or ox-LDL-induced endothelium injury by regulating Akt/mTOR signaling and autophagy.