Validation Data Gallery
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Tested Applications
| Positive WB detected in | A431 cells |
| Positive IHC detected in | human prostate cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 14 publications below |
| IHC | See 4 publications below |
| IF | See 2 publications below |
Product Information
11052-1-AP targets AMD1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | AMD1 fusion protein Ag1525 相同性解析による交差性が予測される生物種 |
| Full Name | adenosylmethionine decarboxylase 1 |
| Calculated molecular weight | 38 kDa, 32 kDa |
| Observed molecular weight | 38-40 kDa |
| GenBank accession number | BC000171 |
| Gene Symbol | AMD1 |
| Gene ID (NCBI) | 262 |
| RRID | AB_2226430 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P17707 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for AMD1 antibody 11052-1-AP | Download protocol |
| IHC protocol for AMD1 antibody 11052-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nature mTORC1-dependent AMD1 regulation sustains polyamine metabolism in prostate cancer.
| ||
Nucleic Acids Res Genome-wide RNAi screen identifies novel players in human 60S subunit biogenesis including key enzymes of polyamine metabolism. | ||
Clin Transl Med AMD1 upregulates hepatocellular carcinoma cells stemness by FTO mediated mRNA demethylation.
| ||
Breast Cancer Res AMD1 promotes breast cancer aggressiveness via a spermidine-eIF5A hypusination-TCF4 axis | ||
iScience The KRAS-G12D mutation induces metabolic vulnerability in B-cell acute lymphoblastic leukemia. | ||
iScience Linkage of methionine addiction, histone lysine hypermethylation, and malignancy. |