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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AMDHD1 Polyclonal antibody

AMDHD1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21171-1-AP

Synonyms

AMDHD1, HMFT1272, Probable imidazolonepropionase

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse skeletal muscle tissue using AMDHD1 Polyclonal antibody (21171-1-AP)

    WB analysis of mouse skeletal muscle using 21171-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21171-1-AP (AMDHD1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using AMDHD1 Polyclonal antibody (21171-1-AP)

    IHC staining of mouse colon using 21171-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 21171-1-AP (AMDHD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using AMDHD1 Polyclonal antibody (21171-1-AP)

    IHC staining of mouse colon using 21171-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 21171-1-AP (AMDHD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse skeletal muscle tissue
Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21171-1-AP targets AMDHD1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AMDHD1 fusion protein Ag15420 相同性解析による交差性が予測される生物種
Full Name amidohydrolase domain containing 1
Calculated molecular weight 426 aa, 47 kDa
Observed molecular weight50 kDa
GenBank accession numberBC029146
Gene symbol AMDHD1
Gene ID (NCBI) 144193
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AMDHD1 is a protein-coding gene that encodes the enzyme imidazolonepropionase. It is involved in the histidine catabolic process and has one potential isoform. The protein of AMDHD1 is located in the cytoplasm, and is expressed in all tissues, mainly in the liver, kidney and adrenal gland.

Protocols

Product Specific Protocols
WB protocol for AMDHD1 antibody 21171-1-APDownload protocol
IHC protocol for AMDHD1 antibody 21171-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols