Validation Data Gallery
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Tested Applications
| Positive WB detected in | 37°C incubated HepG2 cells, HepG2 cells, MCF-7 cells |
| Positive IP detected in | MCF-7 cells |
| Positive IHC detected in | mouse ovary tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
| Positive FC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 9 publications below |
| WB | See 30 publications below |
| IHC | See 1 publications below |
| IF | See 5 publications below |
| IP | See 1 publications below |
| CoIP | See 2 publications below |
Product Information
16675-1-AP targets AMFR/GP78 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | AMFR/GP78 fusion protein Ag10178 相同性解析による交差性が予測される生物種 |
| Full Name | autocrine motility factor receptor |
| Calculated molecular weight | 643 aa, 73 kDa |
| Observed molecular weight | 73 kDa |
| GenBank accession number | BC069197 |
| Gene Symbol | AMFR/GP78 |
| Gene ID (NCBI) | 267 |
| RRID | AB_2226463 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UKV5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
AMFR(Autocrine motility factor receptor) is also named as RNF45,gp78.It is the receptor of Autocrine motility factor(AMF),which is a protein secreted by tumor cells that stimulates tumor motility. AMFR has a potential N-glycosylation site, and several potential O-glycosylation sites(PMID:10456327).In normal fibroblasts,the expression of AMFR appeared to be regulated by cell-cell contact(PMID:7626106).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for AMFR/GP78 antibody 16675-1-AP | Download protocol |
| IHC protocol for AMFR/GP78 antibody 16675-1-AP | Download protocol |
| IF protocol for AMFR/GP78 antibody 16675-1-AP | Download protocol |
| IP protocol for AMFR/GP78 antibody 16675-1-AP | Download protocol |
| FC protocol for AMFR/GP78 antibody 16675-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell BOK Is a Non-canonical BCL-2 Family Effector of Apoptosis Regulated by ER-Associated Degradation.
| ||
Immunity The E3 Ubiquitin Ligase AMFR and INSIG1 Bridge the Activation of TBK1 Kinase by Modifying the Adaptor STING.
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Nat Commun AMFR-mediated Flavivirus NS2A ubiquitination subverts ER-phagy to augment viral pathogenicity | ||
PLoS Biol Reticulon and CLIMP-63 regulate nanodomain organization of peripheral ER tubules. | ||
Brief Bioinform Standigm ASK™: knowledge graph and artificial intelligence platform applied to target discovery in idiopathic pulmonary fibrosis | ||
EMBO Rep MARCH6 and TRC8 facilitate the quality control of cytosolic and tail-anchored proteins.
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