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Target

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Species Reactivity

Species Reactivity

Conjugate

Conjugate

AMH Polyclonal antibody

AMH Polyclonal Antibody for IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 23479-1-AP

Synonyms

Muellerian-inhibiting factor, Muellerian inhibiting factor, Anti-Muellerian hormone (AMH), Anti-Muellerian hormone, anti Mullerian hormone

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human ovary tissue using AMH Polyclonal antibody (23479-1-AP)

    IHC staining of human ovary using 23479-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 23479-1-AP (AMH Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using AMH Polyclonal antibody (23479-1-AP)

    IHC staining of human ovary using 23479-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 23479-1-AP (AMH Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of rat ovary tissue using AMH Polyclonal antibody (23479-1-AP)

    IHC staining of rat ovary using 23479-1-AP

    Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 23479-1-AP (AMH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat ovary tissue using AMH Polyclonal antibody (23479-1-AP)

    IHC staining of rat ovary using 23479-1-AP

    Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 23479-1-AP (AMH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse ovary tissue using AMH Polyclonal antibody (23479-1-AP)

    IF Staining of mouse ovary using 23479-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse ovary tissue using AMH antibody (23479-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse ovary tissue using AMH Polyclonal antibody (23479-1-AP)

    IF Staining of mouse ovary using 23479-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse ovary tissue using AMH antibody (23479-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using AMH Polyclonal antibody (23479-1-AP)

    IF Staining of HeLa using 23479-1-AP

    Immunofluorescent analysis of HeLa cells using 23479-1-AP (AMH antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive IHC detected inhuman ovary tissue, rat ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse ovary tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

23479-1-AP targets AMH in IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AMH fusion protein Ag20166 相同性解析による交差性が予測される生物種
Full Name anti-Mullerian hormone
Calculated molecular weight 59 kDa
GenBank accession numberBC049194
Gene symbol AMH
Gene ID (NCBI) 268
RRIDAB_2879285
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Anti-Müllerian hormone (AMH), also called Müllerian-inhibiting substance (MIS), is a dimeric glycoprotein and a member of the TGF-β family. AMH, produced by the Sertoli cells of the fetal testis, induces the regression of the Müllerian ducts, the anlagen of the female reproductive tract. However, after birth, this sex-dimorphic expression pattern is lost and AMH is also expressed in granulosa cells of growing follicles in the ovary. More recently, AMH has been studied for its role in ovarian folliculogenesis and as a potential marker of ovarian reserve.

Protocols

Product Specific Protocols
IHC protocol for AMH antibody 23479-1-APDownload protocol
IF protocol for AMH antibody 23479-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

ACS Appl Mater Interfaces

A Novel Three-Dimensional Follicle Culture System Decreases Oxidative Stress and Promotes the Prolonged Culture of Human Granulosa Cells

Authors - Xinyang Zhao
View Article