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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AMH Recombinant antibody (84153-5-RR)

    WB analysis using 84153-5-RR (same clone as 84153-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84153-5-RR (AMH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84153-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using AMH Recombinant antibody (84153-5-RR)

    IHC staining of human ovary cancer using 84153-5-RR (same clone as 84153-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84153-5-RR (AMH antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84153-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using AMH Recombinant antibody (84153-5-RR)

    IHC staining of human ovary cancer using 84153-5-RR (same clone as 84153-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84153-5-RR (AMH antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84153-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84153-5-RR

    Affinity and Kinetic Characterization of 84153-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84153-5-RR against Human AMH were performed. The affinity constant is 2.16 nM.

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AMH Recombinant antibody, PBS Only

AMH Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 84153-5-PBS
Clone No.241148C8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Muellerian inhibiting factor, Anti-Muellerian hormone (AMH), Anti-Muellerian hormone, anti Mullerian hormone, Anti muellerian hormone

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84153-5-PBS targets AMH in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen AMH fusion protein Ag5670 相同性解析による交差性が予測される生物種
Full Name anti-Mullerian hormone
Calculated molecular weight 59 kDa
Observed molecular weight60-65 kDa
GenBank accession numberBC049194
Gene Symbol AMH
Gene ID (NCBI) 268
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP03971
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Anti-Müllerian hormone (AMH), also called Müllerian-inhibiting substance (MIS), is a hormone that is best known for its production by fetal testes in mammals and as the inhibitor of Müllerian duct development in males. AMH is also expressed in granulosa cells of preantral and small antral follicles in the ovary. More recently, AMH has been studied for its role in ovarian folliculogenesis and as a potential marker of ovarian reserve. The deduced protein sequence of the AMH monomer contains a 25 amino acid secretion specific signal peptide and a monomeric protein of 535 amino acids that, when glycosylated at two glycosylation sites, has a molecular weight of 65-70 kDa.