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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AMN Polyclonal antibody (32038-1-AP)

    WB analysis using 32038-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32038-1-AP (AMN antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using AMN Polyclonal antibody (32038-1-AP)

    IF Staining of HeLa using 32038-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using AMN antibody (32038-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

AMN Polyclonal antibody

AMN Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 32038-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Protein amnionless, PRO1028, IGS2, Amnionless Homolog, Amnionless

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHuH-7 cells, Jurkat cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32038-1-AP targets AMN in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AMN fusion protein Ag35205 相同性解析による交差性が予測される生物種
Full Name amnionless homolog (mouse)
Calculated molecular weight48 kDa
Observed molecular weight48 kDa
GenBank accession numberNM_030943.3
Gene Symbol AMN
Gene ID (NCBI) 81693
RRIDAB_3670177
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9BXJ7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Amnion associated transmembrane protein (AMN, also known as IGS2, PRO1028, and amnionless) is a 45-50 kDa cysteine-rich type I transmembrane protein that is expressed exclusively in the extra-embryonic visceral endoderm layer during gastrulation (PMID: 17990981; 11279523). It may direct the production of trunk mesoderm derived from the middle streak by acting in the underlying visceral endoderm to modulate a BMP signaling pathway (PMID: 11279523).

Protocols

Product Specific Protocols
WB protocol for AMN antibody 32038-1-APDownload protocol
IF protocol for AMN antibody 32038-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols