Validation Data Gallery
Tested Applications
| Positive WB detected in | human colon tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14694-1-AP targets AMN1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | AMN1 fusion protein Ag6257 相同性解析による交差性が予測される生物種 |
| Full Name | antagonist of mitotic exit network 1 homolog (S. cerevisiae) |
| Calculated molecular weight | 28 kDa |
| Observed molecular weight | 28 kDa |
| GenBank accession number | BC067906 |
| Gene Symbol | AMN1 |
| Gene ID (NCBI) | 196394 |
| RRID | AB_2226647 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IY45 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
AMN1, also known as the antagonist of mitotic exit network 1 homolog, is a protein-coding gene that plays a significant role in regulating cell division and mitotic exit in Saccharomyces cerevisiae (baker's yeast). AMN1 is an atypical F-box protein involved in the regulation of the MEN pathway. It acts as an antagonist by disrupting the formation of the Tem1/Cdc15 complex, which is crucial for mitotic exit. Specifically, AMN1 competes with Cdc15 for binding to Tem1, thereby turning off the MEN pathway. AMN1 is essential for cell separation after cytokinesis. It inhibits the transcription factor Ace2, leading to the downregulation of Ace2 target genes. This inhibition prevents septum cleavage and results in post-mitotic cell separation inhibition, causing cell clumping.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for AMN1 antibody 14694-1-AP | Download protocol |
| IHC protocol for AMN1 antibody 14694-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |