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Validation Data Gallery

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  • Western Blot (WB) analysis of SGC-7901 cells using AMOT Monoclonal antibody (60156-1-Ig)

    WB analysis of SGC-7901 using 60156-1-Ig (same clone as 60156-1-PBS)

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with 60156-1-Ig (AMOT Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60156-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using AMOT Monoclonal antibody (60156-1-Ig)

    WB analysis of HEK-293 using 60156-1-Ig (same clone as 60156-1-PBS)

    WB result of AMOT antibody (60156-1-Ig, 1:1000) with si-Control and si-AMOT transfected HEK293 cells. This data was developed using the same antibody clone with 60156-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using AMOT Monoclonal antibody (60156-1-Ig)

    IF Staining of mouse skeletal muscle using 60156-1-Ig (same clone as 60156-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using AMOT antibody (60156-1-Ig, Clone: 3H7B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60156-1-PBS in a different storage buffer formulation.

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AMOT Monoclonal antibody, PBS Only

AMOT Monoclonal Antibody for WB, IF-P, Indirect ELISA
Cat No. 60156-1-PBS
Clone No.3H7B3

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IF-P, Indirect ELISA

Synonyms

KIAA1071, Angiomotin, 3H7B3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60156-1-PBS targets AMOT in WB, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen peptide 相同性解析による交差性が予測される生物種
Full Name angiomotin
Calculated molecular weight 118 kDa
Observed molecular weight 130 kDa
GenBank accession numberNM_001113490
Gene Symbol AMOT
Gene ID (NCBI) 154796
RRIDAB_10644279
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ4VCS5
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

predominantly in endothelial cells of capillaries as well as larger vessels of the placenta where it may mediate the inhibitory effect of angiostatin on tube formation and the migration of endothelial cells toward growth factors during the formation of new blood vessels. The most abundant expression was found in the placenta and skeletal muscle. AMOT has two isoforms with MW 130 kDa (p130) and 80 kDa (p80). This antibody only recognizes p130.