Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
| IF | See 2 publications below |
Product Information
16871-1-AP targets AMOTL1 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | angiomotin like 1 |
| Calculated molecular weight | 107 kDa |
| Observed molecular weight | 107 kDa |
| GenBank accession number | NM_130847 |
| Gene Symbol | AMOTL1 |
| Gene ID (NCBI) | 154810 |
| RRID | AB_2305483 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IY63 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The protein encoded by this gene is a peripheral membrane protein that is a component of tight junctions or TJs. TJs form an apical junctional structure and act to control paracellular permeability and maintain cell polarity. This protein is related to angiomotin, an angiostatin binding protein that regulates endothelial cell migration and capillary formation.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for AMOTL1 antibody 16871-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Curr Biol Cellular mechanotransduction relies on tension-induced and chaperone-assisted autophagy. | ||
Environ Toxicol Angiomotin-like 1 plays a tumor-promoting role in glioma by enhancing the activation of YAP1 signaling. | ||
Pulm Pharmacol Ther Mitigation of tight junction protein dysfunction in lung microvascular endothelial cells with pitavastatin. | ||
Cell Death Dis SRSF3/AMOTL1 splicing axis promotes the tumorigenesis of nasopharyngeal carcinoma through regulating the nucleus translocation of YAP1 | ||
Int J Biol Macromol Harnessing machine learning and multi-omics to explore tumor evolutionary characteristics and the role of AMOTL1 in prostate cancer |