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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AMPK Beta 2 Monoclonal antibody, PBS Only

AMPK Beta 2 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D5C1

Cat no : 66579-1-PBS

Synonyms

AMPK subunit beta 2, PRKAB2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AMPK Beta 2 Monoclonal antibody (66579-1-Ig)

    WB analysis using 66579-1-Ig (same clone as 66579-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66579-1-Ig (AMPK beta 2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66579-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using AMPK Beta 2 Monoclonal antibody (66579-1-Ig)

    WB analysis of A431 using 66579-1-Ig (same clone as 66579-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 66579-1-Ig (AMPK beta 2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66579-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using AMPK Beta 2 Monoclonal antibody (66579-1-Ig)

    WB analysis of HeLa using 66579-1-Ig (same clone as 66579-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66579-1-Ig (AMPK beta 2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66579-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using AMPK Beta 2 Monoclonal antibody (66579-1-Ig)

    WB analysis of A549 using 66579-1-Ig (same clone as 66579-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 66579-1-Ig (AMPK beta 2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66579-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using AMPK Beta 2 Monoclonal antibody (66579-1-Ig)

    IF Staining of HeLa using 66579-1-Ig (same clone as 66579-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AMPK Beta 2 antibody (66579-1-Ig, Clone: 2D5C1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66579-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66579-1-PBS targets AMPK Beta 2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen AMPK Beta 2 fusion protein Ag5806 相同性解析による交差性が予測される生物種
Full Name protein kinase, AMP-activated, beta 2 non-catalytic subunit
Calculated molecular weight 30 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC053610
Gene symbol AMPK Beta 2
Gene ID (NCBI) 5565
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

AMPK beta 2 is a regulatory subunit of the AMP-activated protein kinase (AMPK), which is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. AMPK beta 2 may be a positive regulator of AMPK activity. It is highly expressed in skeletal muscle and thus may have tissue-specific roles.