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  • Featured Product
  • KD/KO Validated

AMPK Gamma 1 Monoclonal antibody, PBS Only

AMPK Gamma 1 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E11D4

Cat no : 67182-1-PBS

Synonyms

AMPK Gamma 1, AMPK gamma1, AMPK subunit gamma 1, AMPKG, PRKAG1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AMPK Gamma 1 Monoclonal antibody (67182-1-Ig)

    WB analysis using 67182-1-Ig (same clone as 67182-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67182-1-Ig (AMPK gamma 1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67182-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using AMPK Gamma 1 Monoclonal antibody (67182-1-Ig)

    WB analysis of HEK-293 using 67182-1-Ig (same clone as 67182-1-PBS)

    WB result of AMPK Gamma 1 antibody (67182-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AMPK Gamma 1 transfected HEK-293 cells. This data was developed using the same antibody clone with 67182-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67182-1-PBS targets AMPK Gamma 1 in WB, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen AMPK Gamma 1 fusion protein Ag28907 相同性解析による交差性が予測される生物種
Full Name protein kinase, AMP-activated, gamma 1 non-catalytic subunit
Calculated molecular weight 38 kDa
Observed molecular weight 36-38 kDa
GenBank accession numberBC000358
Gene symbol PRKAG1
Gene ID (NCBI) 5571
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Protein kinase, AMP-activated, gamma 1 non-catalytic subunit (PRKAG1, synonyms: AMPKG, MGC8666) is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an catalytic subunit, and non-catalytic and subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and iAMP-activated protein kinase (AMPK) is a highly conserved heterotrimeric serine/threonine kinase widely characterised as a sensor of cellular energetic stress. AMPK is a heterotrimeric complex consisting of a catalytic α-subunit and two regulatory subunits (β and γ). AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. AMPK gamma 1 is one of the gamma regulatory subunits of AMPK.