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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AMPK Gamma 2 Polyclonal antibody, PBS Only

AMPK Gamma 2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12568-1-PBS

Synonyms

AMPK gamma2, PRKAG2, AMPK subunit gamma-2, AMPK subunit gamma 2, AAKG2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AMPK Gamma 2 Polyclonal antibody (12568-1-AP)

    WB analysis using 12568-1-AP (same clone as 12568-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12568-1-AP (AMPK Gamma 2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12568-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using AMPK Gamma 2 Polyclonal antibody (12568-1-AP)

    WB analysis of HeLa using 12568-1-AP (same clone as 12568-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 12568-1-AP (AMPK gamma2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12568-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse eye tissue using AMPK Gamma 2 Polyclonal antibody (12568-1-AP)

    WB analysis of mouse eye using 12568-1-AP (same clone as 12568-1-PBS)

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 12568-1-AP (AMPK gamma2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12568-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using AMPK Gamma 2 Polyclonal antibody (12568-1-AP)

    WB analysis of HEK-293 using 12568-1-AP (same clone as 12568-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12568-1-AP (AMPK gamma2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12568-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using AMPK Gamma 2 Polyclonal antibody (12568-1-AP)

    WB analysis of PC-3 using 12568-1-AP (same clone as 12568-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 12568-1-AP (AMPK gamma2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12568-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using AMPK Gamma 2 Polyclonal antibody (12568-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 12568-1-AP (same clone as 12568-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 12568-1-AP (AMPK Gamma 2 antibody) at dilution of 1:50 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12568-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using AMPK Gamma 2 Polyclonal antibody (12568-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 12568-1-AP (same clone as 12568-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 12568-1-AP (AMPK Gamma 2 antibody) at dilution of 1:50 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12568-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using AMPK Gamma 2 Polyclonal antibody (12568-1-AP)

    IHC staining of human stomach using 12568-1-AP (same clone as 12568-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 12568-1-AP (AMPK Gamma 2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12568-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using AMPK Gamma 2 Polyclonal antibody (12568-1-AP)

    IF Staining of HeLa using 12568-1-AP (same clone as 12568-1-PBS)

    Immunofluorescent analysis of HeLa cells using 12568-1-AP (AMPK gamma2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12568-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12568-1-PBS targets AMPK Gamma 2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AMPK Gamma 2 fusion protein Ag3214 相同性解析による交差性が予測される生物種
Full Name protein kinase, AMP-activated, gamma 2 non-catalytic subunit
Calculated molecular weight 569 aa, 63 kDa
Observed molecular weight 63 kDa
GenBank accession numberBC020540
Gene symbol PRKAG2
Gene ID (NCBI) 51422
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

AMP-activated protein kinase (AMPK) is a heterotrimeric protein composed of a catalytic alpha subunit, a noncatalytic beta subunit, and a noncatalytic regulatory gamma subunit. Various forms of each of these subunits exist, encoded by different genes. AMPK is an important energy-sensing enzyme that monitors cellular energy status and functions by inactivating key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. AMPK gamma2 is a member of the AMPK gamma subunit family. Mutations in this gene have been associated with Wolff-Parkinson-White syndrome, familial hypertrophic cardiomyopathy, and glycogen storage disease of the heart.