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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AMPK Gamma 2 Polyclonal antibody (12568-1-AP)

    WB analysis using 12568-1-AP (same clone as 12568-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12568-1-AP (AMPK Gamma 2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12568-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using AMPK Gamma 2 Polyclonal antibody (12568-1-AP)

    WB analysis using 12568-1-AP (same clone as 12568-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12568-1-AP (AMPK Gamma 2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12568-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using AMPK Gamma 2 Polyclonal antibody (12568-1-AP)

    WB analysis of PC-3 using 12568-1-AP (same clone as 12568-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 12568-1-AP (AMPK gamma2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12568-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using AMPK Gamma 2 Polyclonal antibody (12568-1-AP)

    WB analysis of HeLa using 12568-1-AP (same clone as 12568-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 12568-1-AP (AMPK gamma2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12568-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse eye tissue using AMPK Gamma 2 Polyclonal antibody (12568-1-AP)

    WB analysis of mouse eye using 12568-1-AP (same clone as 12568-1-PBS)

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 12568-1-AP (AMPK gamma2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12568-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using AMPK Gamma 2 Polyclonal antibody (12568-1-AP)

    WB analysis of HEK-293 using 12568-1-AP (same clone as 12568-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12568-1-AP (AMPK gamma2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12568-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using AMPK Gamma 2 Polyclonal antibody (12568-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 12568-1-AP (same clone as 12568-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 12568-1-AP (AMPK Gamma 2 antibody) at dilution of 1:50 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12568-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using AMPK Gamma 2 Polyclonal antibody (12568-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 12568-1-AP (same clone as 12568-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 12568-1-AP (AMPK Gamma 2 antibody) at dilution of 1:50 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12568-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using AMPK Gamma 2 Polyclonal antibody (12568-1-AP)

    IHC staining of human stomach using 12568-1-AP (same clone as 12568-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 12568-1-AP (AMPK Gamma 2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12568-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using AMPK Gamma 2 Polyclonal antibody (12568-1-AP)

    IF Staining of HeLa using 12568-1-AP (same clone as 12568-1-PBS)

    Immunofluorescent analysis of HeLa cells using 12568-1-AP (AMPK gamma2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12568-1-PBS in a different storage buffer formulation.

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AMPK Gamma 2 Polyclonal antibody, PBS Only

AMPK Gamma 2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 12568-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

AMPK gamma2, PRKAG2, AMPK subunit gamma-2, AMPK subunit gamma 2, AAKG2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12568-1-PBS targets AMPK Gamma 2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AMPK Gamma 2 fusion protein Ag3214 相同性解析による交差性が予測される生物種
Full Name protein kinase, AMP-activated, gamma 2 non-catalytic subunit
Calculated molecular weight 569 aa, 63 kDa
Observed molecular weight 63-70 kDa, 38 kDa
GenBank accession numberBC020540
Gene Symbol PRKAG2
Gene ID (NCBI) 51422
RRIDAB_2252960
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UGJ0
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

AMP-activated protein kinase (AMPK) is a heterotrimeric protein composed of a catalytic alpha subunit, a noncatalytic beta subunit, and a noncatalytic regulatory gamma subunit. Various forms of each of these subunits exist, encoded by different genes. AMPK is an important energy-sensing enzyme that monitors cellular energy status and functions by inactivating key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. AMPK gamma2 is a member of the AMPK gamma subunit family. Mutations in this gene have been associated with Wolff-Parkinson-White syndrome, familial hypertrophic cardiomyopathy, and glycogen storage disease of the heart.