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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AMPK alpha Recombinant antibody, PBS Only

AMPK alpha Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4N5

Cat no : 80209-5-PBS

Synonyms

AMPK, AMPK alpha, AMPK subunit alpha 1, AMPK1, AMPKa1, PRKAA1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AMPK alpha Recombinant antibody (80209-5-RR)

    WB analysis using 80209-5-RR (same clone as 80209-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80209-5-RR (AMPK alpha antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80209-5-PBS in a different storage buffer formulation.

  • Affinity experiments of 80209-5-RR

    Affinity experiments of 80209-5-RR

    Biolayer interferometry (BLl) kinetic assays of 80209-5-RR against Human AMPK alpha were performed. The affinity constant is below 1 pM.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80209-5-PBS targets AMPK alpha in WB, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen AMPK alpha fusion protein Ag1367 相同性解析による交差性が予測される生物種
Full Name protein kinase, AMP-activated, alpha 1 catalytic subunit
Calculated molecular weight 63 kDa
GenBank accession numberBC012622
Gene symbol PRKAA1
Gene ID (NCBI) 5562
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.