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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AMPK Beta 1 Polyclonal antibody

AMPK Beta 1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 26907-1-AP

Synonyms

PRKAB1, AMPKb, AMPK β, AMPK subunit beta-1, AMPK subunit beta 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AMPK Beta 1 Polyclonal antibody (26907-1-AP)

    WB analysis using 26907-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26907-1-AP (AMPK Beta 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using AMPK Beta 1 Polyclonal antibody (26907-1-AP)

    WB analysis of Jurkat using 26907-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 26907-1-AP (AMPK beta 1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using AMPK Beta 1 Polyclonal antibody (26907-1-AP)

    WB analysis of NIH/3T3 using 26907-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 26907-1-AP (AMPK beta 1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inA431 cells, Jurkat cells, NIH/3T3 cells, HeLa cells, PC-12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26907-1-AP targets AMPK Beta 1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AMPK Beta 1 fusion protein Ag25534 相同性解析による交差性が予測される生物種
Full Name protein kinase, AMP-activated, beta 1 non-catalytic subunit
Calculated molecular weight 30 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC001056
Gene symbol PRKAB1
Gene ID (NCBI) 5564
RRIDAB_2880679
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AMPK Beta 1 (5'-AMP-activated protein kinase subunit beta-1) is also named as PRKAB1 and AMPK. AMPK, a serine/threonine kinase that exists as a heterotrimer comprised of a catalytic α-subunit and regulatory β- and γ-subunits, has been recognized as a sensor of cellular energy homeostasis (PMID: 21937710). AMPK regulates key metabolic enzymes, cell growth, apoptosis, gene transcription, and protein synthesis (PMID: 12829246). AMPK is an energy sensor and plays an essential role in the control of cellular bioenergetics by responding to various stresses including those that induce changes in the cellular AMP:ATP ratio or modulation in intracellular calcium (PMID: 27812976, PMID: 26616193). Recent studies have shown that AMPK mediates the inhibition of cell proliferation and growth of tumor cells (PMID: 16613876). AMPK also inhibits the expression of Glut1 and glycolysis in Tregs by inhibiting mTORC1 signaling (PMID: 25477880).

Protocols

Product Specific Protocols
WB protocol for AMPK Beta 1 antibody 26907-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols