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Validation Data Gallery

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  • Western Blot (WB) analysis of pig liver tissue using AMT Monoclonal antibody (67532-1-Ig)

    WB analysis of pig liver using 67532-1-Ig

    pig liver tissue were subjected to SDS PAGE followed by western blot with 67532-1-Ig (AMT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat liver tissue using AMT Monoclonal antibody (67532-1-Ig)

    WB analysis of rat liver using 67532-1-Ig

    rat liver tissue were subjected to SDS PAGE followed by western blot with 67532-1-Ig (AMT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using AMT Monoclonal antibody (67532-1-Ig)

    WB analysis of mouse liver using 67532-1-Ig

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 67532-1-Ig (AMT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using AMT Monoclonal antibody (67532-1-Ig)

    IHC staining of human liver cancer using 67532-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67532-1-Ig (AMT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using AMT Monoclonal antibody (67532-1-Ig)

    IHC staining of human liver cancer using 67532-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67532-1-Ig (AMT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using AMT Monoclonal antibody (67532-1-Ig)

    IF Staining of HepG2 using 67532-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using AMT antibody (67532-1-Ig, Clone: 3E7D4 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

AMT Monoclonal antibody

AMT Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67532-1-Ig
Clone No.3E7D4
Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

aminomethyltransferase, AMT, GCE, GCST, GCVT, NKH

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inpig liver tissue, rat liver tissue, mouse liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

67532-1-Ig targets AMT in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen AMT fusion protein Ag26644 相同性解析による交差性が予測される生物種
Full Name aminomethyltransferase
Calculated molecular weight 44 kDa
Observed molecular weight37-44 kDa
GenBank accession numberBC007546
Gene Symbol AMT
Gene ID (NCBI) 275
RRIDAB_2882751
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP48728
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for AMT antibody 67532-1-IgDownload protocol
IHC protocol for AMT antibody 67532-1-IgDownload protocol
IF protocol for AMT antibody 67532-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

iScience

Multiomic landscape of immune pathogenesis in Kimura's disease

Authors - Xunyao Wu
View Article