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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using AMZ2 Polyclonal antibody (16664-1-AP)

    WB analysis of HepG2 using 16664-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16664-1-AP (AMZ2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using AMZ2 Polyclonal antibody (16664-1-AP)

    WB analysis of human brain using 16664-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 16664-1-AP (AMZ2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using AMZ2 Polyclonal antibody (16664-1-AP)

    IHC staining of human liver using 16664-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16664-1-AP (AMZ2 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human testis tissue using AMZ2 Polyclonal antibody (16664-1-AP)

    IHC staining of human testis using 16664-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 16664-1-AP (AMZ2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using AMZ2 Polyclonal antibody (16664-1-AP)

    IHC staining of human skin using 16664-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 16664-1-AP (AMZ2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using AMZ2 Polyclonal antibody (16664-1-AP)

    IHC staining of human skin using 16664-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 16664-1-AP (AMZ2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using AMZ2 Polyclonal antibody (16664-1-AP)

    IHC staining of human brain using 16664-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 16664-1-AP (AMZ2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using AMZ2 Polyclonal antibody (16664-1-AP)

    IHC staining of human brain using 16664-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 16664-1-AP (AMZ2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using AMZ2 Polyclonal antibody (16664-1-AP)

    IHC staining of human liver using 16664-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16664-1-AP (AMZ2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using AMZ2 Polyclonal antibody (16664-1-AP)

    IHC staining of human liver using 16664-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16664-1-AP (AMZ2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using AMZ2 Polyclonal antibody (16664-1-AP)

    IHC staining of human spleen using 16664-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 16664-1-AP (AMZ2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using AMZ2 Polyclonal antibody (16664-1-AP)

    IHC staining of human spleen using 16664-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 16664-1-AP (AMZ2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using AMZ2 Polyclonal antibody (16664-1-AP)

    IHC staining of human ovary using 16664-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 16664-1-AP (AMZ2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using AMZ2 Polyclonal antibody (16664-1-AP)

    IHC staining of human heart using 16664-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 16664-1-AP (AMZ2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using AMZ2 Polyclonal antibody (16664-1-AP)

    IHC staining of human heart using 16664-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 16664-1-AP (AMZ2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using AMZ2 Polyclonal antibody (16664-1-AP)

    IHC staining of human ovary using 16664-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 16664-1-AP (AMZ2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using AMZ2 Polyclonal antibody (16664-1-AP)

    IHC staining of human placenta using 16664-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 16664-1-AP (AMZ2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using AMZ2 Polyclonal antibody (16664-1-AP)

    IHC staining of human placenta using 16664-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 16664-1-AP (AMZ2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using AMZ2 Polyclonal antibody (16664-1-AP)

    IF Staining of HepG2 using 16664-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 16664-1-AP (AMZ2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HepG2 cells using AMZ2 Polyclonal antibody (16664-1-AP)

    FC experiment of HepG2 using 16664-1-AP

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug AMZ2 Polyclonal antibody (16664-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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AMZ2 Polyclonal antibody

AMZ2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 16664-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

EC:3.4.-.-, BM-014, Archeobacterial metalloproteinase-like protein 2, Archaemetzincin-2, Archaemetzincin 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, human brain tissue
Positive IHC detected inhuman liver tissue, human heart tissue, human placenta tissue, human testis tissue, human skin tissue, human brain tissue, human spleen tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16664-1-AP targets AMZ2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AMZ2 fusion protein Ag10141 相同性解析による交差性が予測される生物種
Full Name archaelysin family metallopeptidase 2
Calculated molecular weight 360 aa, 41 kDa
Observed molecular weight 41 kDa
GenBank accession numberBC056271
Gene Symbol AMZ2
Gene ID (NCBI) 51321
RRIDAB_1850886
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86W34
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AMZ2(archaelysin family metallopeptidase 2) is a zinc metalloprotease. Its calculated molecular weight and observed molecular weight are both 41kDa.

Protocols

Product Specific Protocols
WB protocol for AMZ2 antibody 16664-1-APDownload protocol
IHC protocol for AMZ2 antibody 16664-1-APDownload protocol
IF protocol for AMZ2 antibody 16664-1-APDownload protocol
FC protocol for AMZ2 antibody 16664-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols