APC1 Polyclonal antibody

Name: APC1 Polyclonal antibody
Brand: Proteintech
SKU: 21748-1-AP

APC1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 21748-1-AP

Synonyms

TSG 24, Testis-specific gene 24 protein, Mitotic checkpoint regulator, Apc 1, Anaphase-promoting complex subunit 1

Validation Data Gallery

Filter:

AllWBIHC

WB analysis of HeLa using 21748-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 21748-1-AP (APC1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of HT-1080 using 21748-1-AP
HT-1080 cells were subjected to SDS PAGE followed by western blot with 21748-1-AP (APC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 21748-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 21748-1-AP (APC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 21748-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 21748-1-AP (APC1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 21748-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 21748-1-AP (APC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Transfected HEK-293 using 21748-1-AP
Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 21748-1-AP (ANAPC1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of mouse testis using 21748-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21748-1-AP (APC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 21748-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21748-1-AP (APC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 21748-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer using 21748-1-AP (APC1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human cervical cancer using 21748-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer using 21748-1-AP (APC1 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human stomach using 21748-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach using 21748-1-AP (APC1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human stomach using 21748-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach using 21748-1-AP (APC1 antibody) at dilution of 1:50 (under 40x lens).

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Cat No. 21748-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, HEK-293 cells, HT-1080 cells, K-562 cells, mouse brain tissue, Transfected HEK-293 cells
Positive IHC detected in	mouse testis tissue, human cervical cancer tissue, human stomach tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 4 publications below

Product Information

21748-1-AP targets APC1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	APC1 fusion protein Ag13399 相同性解析による交差性が予測される生物種
Full Name	anaphase promoting complex subunit 1
Calculated molecular weight	1944 aa, 217 kDa
Observed molecular weight	200-210 kDa
GenBank accession number	BC104902
Gene symbol	APC1
Gene ID (NCBI)	64682
RRID	AB_10733241
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for APC1 antibody 21748-1-AP	Download protocol
IHC protocol for APC1 antibody 21748-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Commun DNA replication initiation factor RECQ4 possesses a role in antagonizing DNA replication initiation Authors - Xiaohua Xu View Article
human	WB	Oncotarget Screening key microRNAs for castration-resistant prostate cancer based on miRNA/mRNA functional synergistic network. Authors - Jin Zhu View Article
human	WB	Sci Rep Reciprocal interaction between SIRT6 and APC/C regulates genomic stability. Authors - Helin Wang View Article
human	WB	Cell Rep TRPS1 drives heterochromatic origin refiring and cancer genome evolution. Authors - Jianguo Yang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

APC1 Polyclonal antibody

APC1 Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis of HeLa using 21748-1-AP

WB analysis of HT-1080 using 21748-1-AP

WB analysis of HEK-293 using 21748-1-AP

WB analysis of mouse brain using 21748-1-AP

WB analysis of K-562 using 21748-1-AP

WB analysis of Transfected HEK-293 using 21748-1-AP

IHC staining of mouse testis using 21748-1-AP

IHC staining of mouse testis using 21748-1-AP

IHC staining of human cervical cancer using 21748-1-AP

IHC staining of human cervical cancer using 21748-1-AP

IHC staining of human stomach using 21748-1-AP

IHC staining of human stomach using 21748-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

DNA replication initiation factor RECQ4 possesses a role in antagonizing DNA replication initiation

Screening key microRNAs for castration-resistant prostate cancer based on miRNA/mRNA functional synergistic network.

Reciprocal interaction between SIRT6 and APC/C regulates genomic stability.

TRPS1 drives heterochromatic origin refiring and cancer genome evolution.