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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ANAPC10 Polyclonal antibody

ANAPC10 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15788-1-AP

Synonyms

DOC1, APC10, Anaphase-promoting complex subunit 10, ANAPC 10

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ANAPC10 Polyclonal antibody (15788-1-AP)

    WB analysis using 15788-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15788-1-AP (ANAPC10 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human heart tissue using ANAPC10 Polyclonal antibody (15788-1-AP)

    IHC staining of human heart using 15788-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15788-1-AP (ANAPC10 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using ANAPC10 Polyclonal antibody (15788-1-AP)

    IHC staining of human heart using 15788-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15788-1-AP (ANAPC10 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15788-1-AP targets ANAPC10 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ANAPC10 fusion protein Ag8487 相同性解析による交差性が予測される生物種
Full Name anaphase promoting complex subunit 10
Calculated molecular weight 185 aa, 21 kDa
Observed molecular weight20 kDa
GenBank accession numberBC005217
Gene symbol ANAPC10
Gene ID (NCBI) 10393
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Anaphase-promoting complex subunit 10 (Anapc10) is a component of the promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase.Anapc10 recognizes ubiquitination-mediated degradation of substrates, and is expressed predominantly in the cytoplasm of spermatogonial and leptoblast cells.

Protocols

Product Specific Protocols
WB protocol for ANAPC10 antibody 15788-1-APDownload protocol
IHC protocol for ANAPC10 antibody 15788-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols