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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ANAPC10 Recombinant antibody

ANAPC10 Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240492E7

Cat no : 83570-4-RR

Synonyms

DOC1, APC10, Anaphase-promoting complex subunit 10, ANAPC 10, 240492E7

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ANAPC10 Recombinant antibody (83570-4-RR)

    IF Staining of HeLa using 83570-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ANAPC10 antibody (83570-4-RR, Clone: 240492E7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83570-4-RR

    Affinity and Kinetic Characterization of 83570-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83570-4-RR against Human ANAPC10 were performed. The affinity constant is 0.141 nM.

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Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83570-4-RR is ANAPC10 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ANAPC10 fusion protein Ag8487 相同性解析による交差性が予測される生物種
Full Name anaphase promoting complex subunit 10
Calculated molecular weight 185 aa, 21 kDa
GenBank accession numberBC005217
Gene symbol ANAPC10
Gene ID (NCBI) 10393
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The anaphase promoting complex/cyclosome (APC/C) is a cell cycle-regulated multimeric E3 ubiquitin ligase assembled from 13 individual subunits. The anaphase promoting complex subunit 10 (APC10) is a core subunit of APC/C that is highly conserved in humans. APC10 genetically and physically interacts with a series of subunits of the APC/C and is necessary for the ubiquitination activity of APC/C by enhancing the affinity of the APC/C for its substrate.

Protocols

Product Specific Protocols
IF protocol for ANAPC10 antibody 83570-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols