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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ANGPTL3 Polyclonal antibody

ANGPTL3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11964-1-AP

Synonyms

ANG 5, Angiopoietin 5, angiopoietin like 3, Angiopoietin like protein 3, Angiopoietin related protein 3, ANGPT5, ANGPTL3

Validation Data Gallery

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  • Western Blot (WB) analysis of A375 cells using ANGPTL3 Polyclonal antibody (11964-1-AP)

    WB analysis of A375 using 11964-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 11964-1-AP (ANGPTL3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using ANGPTL3 Polyclonal antibody (11964-1-AP)

    WB analysis of mouse liver using 11964-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 11964-1-AP (ANGPTL3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using ANGPTL3 Polyclonal antibody (11964-1-AP)

    IHC staining of human liver using 11964-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11964-1-AP (ANGPTL3 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using ANGPTL3 Polyclonal antibody (11964-1-AP)

    IHC staining of human liver using 11964-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11964-1-AP (ANGPTL3 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

Positive WB detected inA375 cells, mouse liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

11964-1-AP targets ANGPTL3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ANGPTL3 fusion protein Ag2573 相同性解析による交差性が予測される生物種
Full Name angiopoietin-like 3
Calculated molecular weight 460 aa, 54 kDa
Observed molecular weight 54 kDa
GenBank accession numberBC058287
Gene symbol ANGPTL3
Gene ID (NCBI) 27329
RRIDAB_874076
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ANGPTL3 belongs to the angiopoietin-like protein family. ANGPTL3 is mainly synthesized by liver cells and is notably expressed in kidney podocytes. Accumulating evidences have revealed that ANGPTL3 plays a critical role in both biological processes, such as lipid metabolism, angiogenesis and haematopoietic function and pathological changes, including atherosclerosis, carcinogenesis, nephrotic syndrome, diabetes, liver diseases and so on. Thus, ANGPTL3 may serve as a potential biomarker in these diseases.

Protocols

Product Specific Protocols
WB protocol for ANGPTL3 antibody 11964-1-APDownload protocol
IHC protocol for ANGPTL3 antibody 11964-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,ratWB

Invest Ophthalmol Vis Sci

PMID: 30128492

Authors - Na Wang
View Article
humanWB

Cancer Sci

Screening of novel serum biomarkers for gastric cancer in coastal populations using a protein microarray

Authors - Yongdong Yi
View Article