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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ANGPTL4 Monoclonal antibody

ANGPTL4 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1D2H6

Cat no : 67577-1-Ig

Synonyms

Angiopoietin-related protein 4, Angiopoietin-like protein 4, Angiopoietin related protein 4, Angiopoietin like protein 4, angiopoietin like 4

Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using ANGPTL4 Monoclonal antibody (67577-1-Ig)

    WB analysis of human placenta using 67577-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67577-1-Ig (ANGPTL4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HT-29 cells using ANGPTL4 Monoclonal antibody (67577-1-Ig)

    WB analysis of HT-29 using 67577-1-Ig

    HT-29 cells were subjected to SDS PAGE followed by western blot with 67577-1-Ig (ANGPTL4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using ANGPTL4 Monoclonal antibody (67577-1-Ig)

    WB analysis of A549 using 67577-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 67577-1-Ig (ANGPTL4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using ANGPTL4 Monoclonal antibody (67577-1-Ig)

    WB analysis of COLO 320 using 67577-1-Ig

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 67577-1-Ig (ANGPTL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Caco-2 cells using ANGPTL4 Monoclonal antibody (67577-1-Ig)

    WB analysis of Caco-2 using 67577-1-Ig

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 67577-1-Ig (ANGPTL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HT-29 cells using ANGPTL4 Monoclonal antibody (67577-1-Ig)

    WB analysis of HT-29 using 67577-1-Ig

    HT-29 cells were subjected to SDS PAGE followed by western blot with 67577-1-Ig (ANGPTL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using ANGPTL4 Monoclonal antibody (67577-1-Ig)

    WB analysis of mouse brain using 67577-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 67577-1-Ig (ANGPTL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using ANGPTL4 Monoclonal antibody (67577-1-Ig)

    IF Staining of NIH/3T3 using 67577-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using ANGPTL4 antibody (67577-1-Ig, Clone: 1D2H6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inhuman placenta tissue, COLO 320 cells, Caco-2 cells, HT-29 cells, mouse brain tissue, A549 cells
Positive IF/ICC detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67577-1-Ig targets ANGPTL4 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen ANGPTL4 fusion protein Ag30070 相同性解析による交差性が予測される生物種
Full Name angiopoietin-like 4
Calculated molecular weight 45 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC023647
Gene symbol ANGPTL4
Gene ID (NCBI) 51129
RRIDAB_2882790
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Angiopoietin-like protein 4 (ANGPTL4), a member of the angiopoietin-like protein subfamily, is a secretory protein that functions as an important modulator of glucose and lipid metabolism. ANGPTL4 inhibits lipoprotein lipase (LPL)-dependent lipolysis thereby limiting the uptake of free fatty acids. Overexpression of ANGPTL4 results in hypertriglyceridemia, while ANGPTL4 deficiency suppresses foam formation in macrophages and protects against atherosclerosis. ANGPTL4 may play a role in several cancers and it also has been shown to prevent the metastatic process by inhibiting vascular activity as well as tumor cell motility and invasiveness. Decreased expression of this protein has been associated with type 2 diabetes. ANGPTL4 has three isoforms, 45 kDa, 41 kDa and 27 kDa.

Protocols

Product Specific Protocols
WB protocol for ANGPTL4 antibody 67577-1-IgDownload protocol
IF protocol for ANGPTL4 antibody 67577-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

BMC Biol

Molecular, cellular and neurological consequences of infection by the neglected human pathogen Nocardia

Authors - Xingzhao Ji
View Article