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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ANK1 Polyclonal antibody (23938-1-AP)

    WB analysis using 23938-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23938-1-AP (ANK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using ANK1 Polyclonal antibody (23938-1-AP)

    IHC staining of mouse cerebellum using 23938-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 23938-1-AP (ANK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using ANK1 Polyclonal antibody (23938-1-AP)

    IHC staining of mouse cerebellum using 23938-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 23938-1-AP (ANK1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat cerebellum tissue using ANK1 Polyclonal antibody (23938-1-AP)

    IHC staining of rat cerebellum using 23938-1-AP

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 23938-1-AP (ANK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat cerebellum tissue using ANK1 Polyclonal antibody (23938-1-AP)

    IHC staining of rat cerebellum using 23938-1-AP

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 23938-1-AP (ANK1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using ANK1 Polyclonal antibody (23938-1-AP)

    IHC staining of human lymphoma using 23938-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 23938-1-AP (ANK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using ANK1 Polyclonal antibody (23938-1-AP)

    IHC staining of human stomach cancer using 23938-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 23938-1-AP (ANK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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ANK1 Polyclonal antibody

ANK1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 23938-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Ankyrin 1, Ankyrin-1, Ankyrin R, ANK-1, ANK 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration:  150 μL, 750 ug/ml
20 μL, 750 ug/ml
150 μL, 750 ug/ml

¥67,000/ 150 μL

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inK-562 cells, mouse liver tissue
Positive IHC detected inhuman stomach cancer tissue, human lymphoma tissue, mouse cerebellum tissue, rat cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23938-1-AP targets ANK1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ANK1 fusion protein Ag21041 相同性解析による交差性が予測される生物種
Full Name ankyrin 1, erythrocytic
Calculated molecular weight 155 aa, 18 kDa
Observed molecular weight 190-250 kDa
GenBank accession numberBC030957
Gene Symbol ANK1
Gene ID (NCBI) 286
RRIDAB_3085717
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDP16157
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ankyrin-1 (ANK1) is also named as Ankyrin-R and Erythrocyte ankyrin. The stability and shape of the erythrocyte membrane is provided by the ankyrin-1 complex (PMID:18440852). ANK1 can attache integral membrane proteins to cytoskeletal elements. And it binds to the erythrocyte membrane protein band 4.2, to Na-K ATPase, to the lymphocyte membrane protein GP85, and to the cytoskeletal proteins fodrin, tubulin, vimentin and desmin.(PMID:12456646).

Protocols

Product Specific Protocols
WB protocol for ANK1 antibody 23938-1-APDownload protocol
IHC protocol for ANK1 antibody 23938-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols