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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ANK2 Polyclonal antibody (31080-1-AP)

    WB analysis using 31080-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31080-1-AP (ANK2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using ANK2 Polyclonal antibody (31080-1-AP)

    IF Staining of mouse heart using 31080-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using ANK2 antibody (31080-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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ANK2 Polyclonal antibody

ANK2 Polyclonal Antibody for WB, IF-P, ELISA
Cat No. 31080-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-P, ELISA

Synonyms

Ankyrin 2, ANK 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, mouse heart tissue, rat heart tissue
Positive IF-P detected inmouse heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31080-1-AP targets ANK2 in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ANK2 fusion protein Ag34642 相同性解析による交差性が予測される生物種
Full Name ankyrin 2, neuronal
Calculated molecular weight434 kDa
Observed molecular weight250-260 kDa
GenBank accession numberNM_001148
Gene Symbol ANK2
Gene ID (NCBI) 287
RRIDAB_3669845
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ01484
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The Ankyrins, belonging to the adapter protein family, consist of Ankyrin-R (ANK1), Ankyrin-B (ANK2), and Ankyrin-G (ANK3). Initially, ANK2 was considered as a key cytoskeletal-associated protein in the brain. Besides, it was discovered as a pivotal adaptor and scaffolding protein in the heart. Remarkably, recent studies have demonstrated that Ankyrins contribute to the modulation of various biological activities in cancers. (PMID: 36539782)

Protocols

Product Specific Protocols
WB protocol for ANK2 antibody 31080-1-APDownload protocol
IF protocol for ANK2 antibody 31080-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols