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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ANK3 Polyclonal antibody

ANK3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 27980-1-AP

Synonyms

ANK 3, ANK3, Ankyrin 3, ANKYRIN G, FLJ45464

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ANK3 Polyclonal antibody (27980-1-AP)

    WB analysis using 27980-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27980-1-AP (ANK3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ANK3 Polyclonal antibody (27980-1-AP)

    IHC staining of human breast cancer using 27980-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27980-1-AP (ANK3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ANK3 Polyclonal antibody (27980-1-AP)

    IHC staining of human breast cancer using 27980-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27980-1-AP (ANK3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ANK3 Polyclonal antibody (27980-1-AP)

    IHC staining of mouse brain using 27980-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27980-1-AP (ANK3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ANK3 Polyclonal antibody (27980-1-AP)

    IHC staining of mouse brain using 27980-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27980-1-AP (ANK3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using ANK3 Polyclonal antibody (27980-1-AP)

    IF Staining of PC-3 using 27980-1-AP

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using ANK3 antibody (27980-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman breast cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

27980-1-AP targets ANK3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ANK3 fusion protein Ag27635 相同性解析による交差性が予測される生物種
Full Name ankyrin 3, node of Ranvier (ankyrin G)
Calculated molecular weight 480 kDa
Observed molecular weight160 kDa, 204 kDa
GenBank accession numberNM_001149
Gene symbol ANK3
Gene ID (NCBI) 288
RRIDAB_2881028
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ANK3 is associated with neurodevelopment and neuronal function. It has been reported that ANK3 plays a key role in bipolar disorder. ANK3 is expressed in brain at a high level. There are several isoforms of ANK3 associated with different tissue expression and function. The immune region we selected can recognize 480 kDa, 204 kDa, 202 kDa and 111 kDa protein and 27980-1-AP detects 204 kDa isoform in SDS-PAGE. (PMID: 27217151, 28687526, 30297702, 28109561, 30046097)

Protocols

Product Specific Protocols
WB protocol for ANK3 antibody 27980-1-APDownload protocol
IHC protocol for ANK3 antibody 27980-1-APDownload protocol
IF protocol for ANK3 antibody 27980-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Immunol

A comprehensive cuproptosis score and associated gene signatures reveal prognostic and immunological features of idiopathic pulmonary fibrosis

Authors - Chuanqing Jing
View Article