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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ANKIB1 Polyclonal antibody (30467-1-AP)

    WB analysis using 30467-1-AP (same clone as 30467-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30467-1-AP (ANKIB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30467-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using ANKIB1 Polyclonal antibody (30467-1-AP)

    IP experiment of HeLa using 30467-1-AP (same clone as 30467-1-PBS)

    IP result of anti-ANKIB1 (IP:30467-1-AP, 4ug; Detection:30467-1-AP 1:4000) with HeLa cells lysate 1560 ug. This data was developed using the same antibody clone with 30467-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using ANKIB1 Polyclonal antibody (30467-1-AP)

    IHC staining of mouse kidney using 30467-1-AP (same clone as 30467-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30467-1-AP (ANKIB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30467-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using ANKIB1 Polyclonal antibody (30467-1-AP)

    IHC staining of mouse kidney using 30467-1-AP (same clone as 30467-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30467-1-AP (ANKIB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30467-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse placenta tissue using ANKIB1 Polyclonal antibody (30467-1-AP)

    IHC staining of mouse placenta using 30467-1-AP (same clone as 30467-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 30467-1-AP (ANKIB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30467-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse placenta tissue using ANKIB1 Polyclonal antibody (30467-1-AP)

    IHC staining of mouse placenta using 30467-1-AP (same clone as 30467-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 30467-1-AP (ANKIB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30467-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ANKIB1 Polyclonal antibody (30467-1-AP)

    IHC staining of mouse brain using 30467-1-AP (same clone as 30467-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30467-1-AP (ANKIB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30467-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using ANKIB1 Polyclonal antibody (30467-1-AP)

    IF Staining of U2OS using 30467-1-AP (same clone as 30467-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using ANKIB1 antibody (30467-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30467-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using ANKIB1 Polyclonal antibody (30467-1-AP)

    IF Staining of A431 using 30467-1-AP (same clone as 30467-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using ANKIB1 antibody (30467-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30467-1-PBS in a different storage buffer formulation.

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ANKIB1 Polyclonal antibody, PBS Only

ANKIB1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 30467-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30467-1-PBS targets ANKIB1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ANKIB1 fusion protein Ag33126 相同性解析による交差性が予測される生物種
Full Name ankyrin repeat and IBR domain containing 1
Calculated molecular weight122 kDa
Observed molecular weight 122 kDa
GenBank accession numberNM_019004.1
Gene Symbol ANKIB1
Gene ID (NCBI) 54467
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P2G1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Ankyrin repeat and IBR domain-containing protein 1 (ANKIB1) is also named as KIAA1386, and belongs to the RBR family. ANKIB1 might act as an E3 ubiquitin-protein ligase, or as part of E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates. The common biomarkers found within the H and EC region of brain are ZNF621, SLC25A46, RAE1, and ANKIB1. Among these biomarkers, RAE1, ANKIB1, and SLC25A46 have been reported to be prominently involved in several neurodegenerative disorders (PMID:33878365). ANKIB1 is also found to be associated with Cerebral cavernous malformations (PMID:20798775).