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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ANKRD1 Monoclonal antibody, PBS Only

ANKRD1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat, rabbit, pig

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1F11F1

Cat no : 67775-1-PBS

Synonyms

ALRP, ANKRD1, bA320F15.2, C 193, C193, Cardiac ankyrin repeat protein, CARP, CVARP, HA1A2, MCARP

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse heart tissue using ANKRD1 Monoclonal antibody (67775-1-Ig)

    WB analysis of mouse heart using 67775-1-Ig (same clone as 67775-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 67775-1-Ig (ANKRD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67775-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rabbit heart tissue using ANKRD1 Monoclonal antibody (67775-1-Ig)

    WB analysis of rabbit heart using 67775-1-Ig (same clone as 67775-1-PBS)

    rabbit heart tissue were subjected to SDS PAGE followed by western blot with 67775-1-Ig (ANKRD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67775-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig heart tissue using ANKRD1 Monoclonal antibody (67775-1-Ig)

    WB analysis of pig heart using 67775-1-Ig (same clone as 67775-1-PBS)

    pig heart tissue were subjected to SDS PAGE followed by western blot with 67775-1-Ig (ANKRD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67775-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using ANKRD1 Monoclonal antibody (67775-1-Ig)

    IF Staining of A431 using 67775-1-Ig (same clone as 67775-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using ANKRD1 antibody (67775-1-Ig, Clone: 1F11F1 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67775-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67775-1-PBS targets ANKRD1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.

Tested Reactivity Human, mouse, rat, rabbit, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen ANKRD1 fusion protein Ag29974 相同性解析による交差性が予測される生物種
Full Name ankyrin repeat domain 1 (cardiac muscle)
Calculated molecular weight 319 aa, 36 kDa
GenBank accession numberBC018667
Gene symbol ANKRD1
Gene ID (NCBI) 27063
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

Ankyrin repeat domain-containing protein 1(ANKRD1) belongs to the conserved muscle ankyrin repeat protein(MARP) family, whose expression is induced in response to physiologic stress, injury and hypertrophy. Thus its expression mainly in activated vascular endothelial cells. ANKRD1 may regulate SMC proliferation through the CDKN1A pathway. It functions as a nuclear transcription repressor that regulate cardiac gene's expression