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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ANKRD13A Polyclonal antibody (23998-1-AP)

    WB analysis using 23998-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23998-1-AP (ANKRD13A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using ANKRD13A Polyclonal antibody (23998-1-AP)

    WB analysis of HepG2 using 23998-1-AP

    WB result of ANKRD13A antibody (23998-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ANKRD13A transfected HepG2 cells. The 100 kDa band may be a product of binding with ubiquitin.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

  • Featured Product
  • KD/KO Validated

ANKRD13A Polyclonal antibody

ANKRD13A Polyclonal Antibody for WB, ELISA
Cat No. 23998-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Synonyms

Protein KE03, Ankyrin repeat domain-containing protein 13A, ANKRD13

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, Jurkat cells, LNCaP cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23998-1-AP targets ANKRD13A in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ANKRD13A fusion protein Ag21200 相同性解析による交差性が予測される生物種
Full Name ankyrin repeat domain 13A
Calculated molecular weight 590 aa, 68 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC032833
Gene Symbol ANKRD13A
Gene ID (NCBI) 88455
RRIDAB_2879397
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ8IZ07
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ANKRD13A, also named ANKRD13, a 590 amino acid protein, which contains two ANK repeats and four UIM (ubiquitin-interacting motif) repeats. ANKRD13A localizes in the cell membrane. ANKRD13A as ubiquitin-binding protein that specifically recognizes and binds 'Lys-63'-linked ubiquitin, not bind 'Lys-48'-linked ubiquitin. ANKRD13A positively regulates the internalization of ligand-activated EGFR by binding to the Ub moiety of ubiquitinated EGFR at the cell membrane.

Protocols

Product Specific Protocols
WB protocol for ANKRD13A antibody 23998-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

J Biol Chem

CYYR1 promotes the degradation of the E3 ubiquitin ligase WWP1 and is associated with favorable prognosis in breast cancer

Authors - Tiphaine Perron
View Article