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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ANKRD13A Polyclonal antibody, PBS Only

ANKRD13A Polyclonal Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

Cat no : 23998-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ANKRD13A Polyclonal antibody (23998-1-AP)

    WB analysis using 23998-1-AP (same clone as 23998-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 23998-1-AP (ANKRD13A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23998-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ANKRD13A Polyclonal antibody (23998-1-AP)

    WB analysis using 23998-1-AP (same clone as 23998-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 23998-1-AP (ANKRD13A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23998-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using ANKRD13A Polyclonal antibody (23998-1-AP)

    WB analysis of HepG2 using 23998-1-AP (same clone as 23998-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 23998-1-AP (ANKRD13A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23998-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

23998-1-PBS targets ANKRD13A in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ANKRD13A fusion protein Ag21200 相同性解析による交差性が予測される生物種
Full Name ankyrin repeat domain 13A
Calculated molecular weight 590 aa, 68 kDa
Observed molecular weight 80-90 kDa
GenBank accession numberBC032833
Gene symbol ANKRD13A
Gene ID (NCBI) 88455
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ANKRD13A, also named as ANKRD13, a 590 amino acid protein, which contains two ANK repeats and four UIM (ubiquitin-interacting motif) repeats. ANKRD13A localizes in the cell membrane. ANKRD13A as ubiquitin-binding protein that specifically recognizes and binds 'Lys-63'-linked ubiquitin, not bind 'Lys-48'-linked ubiquitin. ANKRD13A positively regulates the internalization of ligand-activated EGFR by binding to the Ub moiety of ubiquitinated EGFR at the cell membrane.