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  • Featured Product
  • KD/KO Validated

ANKRD13A Polyclonal antibody, PBS Only

ANKRD13A Polyclonal Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

Cat no : 23998-1-PBS

Synonyms

Protein KE03, Ankyrin repeat domain-containing protein 13A, ANKRD13

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ANKRD13A Polyclonal antibody (23998-1-AP)

    WB analysis using 23998-1-AP (same clone as 23998-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 23998-1-AP (ANKRD13A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23998-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using ANKRD13A Polyclonal antibody (23998-1-AP)

    WB analysis of HepG2 using 23998-1-AP (same clone as 23998-1-PBS)

    WB result of ANKRD13A antibody (23998-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ANKRD13A transfected HepG2 cells. The 100 kDa band may be a product of binding with ubiquitin. This data was developed using the same antibody clone with 23998-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

23998-1-PBS targets ANKRD13A in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ANKRD13A fusion protein Ag21200 相同性解析による交差性が予測される生物種
Full Name ankyrin repeat domain 13A
Calculated molecular weight 590 aa, 68 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC032833
Gene symbol ANKRD13A
Gene ID (NCBI) 88455
RRIDAB_2879397
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ANKRD13A, also named ANKRD13, a 590 amino acid protein, which contains two ANK repeats and four UIM (ubiquitin-interacting motif) repeats. ANKRD13A localizes in the cell membrane. ANKRD13A as ubiquitin-binding protein that specifically recognizes and binds 'Lys-63'-linked ubiquitin, not bind 'Lys-48'-linked ubiquitin. ANKRD13A positively regulates the internalization of ligand-activated EGFR by binding to the Ub moiety of ubiquitinated EGFR at the cell membrane.