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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ANKRD16 Polyclonal antibody

ANKRD16 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24334-1-AP

Synonyms

Ankyrin repeat domain-containing protein 16, ankyrin repeat domain 16

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using ANKRD16 Polyclonal antibody (24334-1-AP)

    WB analysis of HeLa using 24334-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24334-1-AP (ANKRD16 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using ANKRD16 Polyclonal antibody (24334-1-AP)

    WB analysis of PC-3 using 24334-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 24334-1-AP (ANKRD16 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using ANKRD16 Polyclonal antibody (24334-1-AP)

    WB analysis of PC-3 using 24334-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 24334-1-AP (ANKRD16 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using ANKRD16 Polyclonal antibody (24334-1-AP)

    WB analysis of mouse liver using 24334-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 24334-1-AP (ANKRD16 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using ANKRD16 Polyclonal antibody (24334-1-AP)

    IHC staining of human ovary cancer using 24334-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 24334-1-AP (ANKRD16 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ANKRD16 Polyclonal antibody (24334-1-AP)

    IF Staining of HeLa using 24334-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ANKRD16 antibody (24334-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

Positive WB detected inHeLa cells, mouse liver tissue, PC-3 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24334-1-AP targets ANKRD16 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ANKRD16 fusion protein Ag19369 相同性解析による交差性が予測される生物種
Full Name ankyrin repeat domain 16
Calculated molecular weight 361 aa, 39 kDa
Observed molecular weight 39-45 kDa
GenBank accession numberBC062346
Gene symbol ANKRD16
Gene ID (NCBI) 54522
RRIDAB_2879499
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ANKRD16, a vertebrate-specific protein that contains ankyrin repeats, binds directly to the catalytic domain of AlaRS. Serine that is misactivated by AlaRS is captured by the lysine side chains of ANKRD16, which prevents the charging of serine adenylates to tRNAAla and precludes serine misincorporation in nascent peptides. The deletion of Ankrd16 in the brains of Aarssti/sti mice causes widespread protein aggregation and neuron loss (PMID: 29769718).

Protocols

Product Specific Protocols
WB protocol for ANKRD16 antibody 24334-1-APDownload protocol
IHC protocol for ANKRD16 antibody 24334-1-APDownload protocol
IF protocol for ANKRD16 antibody 24334-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols