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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ANKRD17 Polyclonal antibody

ANKRD17 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27701-1-AP

Synonyms

NY BR 16, KIAA0697, GTAR, ankyrin repeat domain 17

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ANKRD17 Polyclonal antibody (27701-1-AP)

    WB analysis using 27701-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27701-1-AP (ANKRD17 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using ANKRD17 Polyclonal antibody (27701-1-AP)

    IHC staining of human ovary cancer using 27701-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27701-1-AP (ANKRD17 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27701-1-AP targets ANKRD17 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ANKRD17 fusion protein Ag26767 相同性解析による交差性が予測される生物種
Full Name ankyrin repeat domain 17
Calculated molecular weight 274 kDa
Observed molecular weight270-300 kDa
GenBank accession numberNM_001286771
Gene symbol ANKRD17
Gene ID (NCBI) 26057
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ANKRD17 belongs to the family of ankyrin repeat-containing proteins, and contains two distinct arrays of ankyrin repeats in its amino-terminal region. It also contains a nuclear export signal, nuclear localization signal, and a cyclin-binding RXL motif. Localization of this protein to the nucleus has been shown experimentally, and interactions between this protein and cyclin-dependent kinase 2 have been observed.

Protocols

Product Specific Protocols
WB protocol for ANKRD17 antibody 27701-1-APDownload protocol
IHC protocol for ANKRD17 antibody 27701-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols