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  • Featured Product
  • KD/KO Validated

ANKRD2 Polyclonal antibody, PBS Only

ANKRD2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11821-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse skeletal muscle tissue using ANKRD2 Polyclonal antibody (11821-1-AP)

    WB analysis of mouse skeletal muscle using 11821-1-AP (same clone as 11821-1-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11821-1-AP (ANKRD2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11821-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human skeletal muscle tissue using ANKRD2 Polyclonal antibody (11821-1-AP)

    WB analysis of human skeletal muscle using 11821-1-AP (same clone as 11821-1-PBS)

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11821-1-AP (ANKRD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11821-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse skeletal muscle tissue using ANKRD2 Polyclonal antibody (11821-1-AP)

    IP experiment of mouse skeletal muscle using 11821-1-AP (same clone as 11821-1-PBS)

    IP result of anti-ANKRD2 (IP:11821-1-AP, 4ug; Detection:11821-1-AP 1:500) with mouse skeletal muscle tissue lysate 3600ug. This data was developed using the same antibody clone with 11821-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using ANKRD2 Polyclonal antibody (11821-1-AP)

    IHC staining of human kidney using 11821-1-AP (same clone as 11821-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 11821-1-AP (ANKRD2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 11821-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using ANKRD2 Polyclonal antibody (11821-1-AP)

    IHC staining of human kidney using 11821-1-AP (same clone as 11821-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 11821-1-AP (ANKRD2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 11821-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using ANKRD2 Polyclonal antibody (11821-1-AP)

    IHC staining of human testis using 11821-1-AP (same clone as 11821-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 11821-1-AP (ANKRD2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 11821-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using ANKRD2 Polyclonal antibody (11821-1-AP)

    IHC staining of human testis using 11821-1-AP (same clone as 11821-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 11821-1-AP (ANKRD2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 11821-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin tissue using ANKRD2 Polyclonal antibody (11821-1-AP)

    IHC staining of human skin using 11821-1-AP (same clone as 11821-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin using 11821-1-AP (ANKRD2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 11821-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin tissue using ANKRD2 Polyclonal antibody (11821-1-AP)

    IHC staining of human skin using 11821-1-AP (same clone as 11821-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin using 11821-1-AP (ANKRD2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 11821-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using ANKRD2 Polyclonal antibody (11821-1-AP)

    IHC staining of human brain using 11821-1-AP (same clone as 11821-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 11821-1-AP (ANKRD2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 11821-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using ANKRD2 Polyclonal antibody (11821-1-AP)

    IHC staining of human brain using 11821-1-AP (same clone as 11821-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 11821-1-AP (ANKRD2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 11821-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using ANKRD2 Polyclonal antibody (11821-1-AP)

    IHC staining of human lung using 11821-1-AP (same clone as 11821-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung using 11821-1-AP (ANKRD2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 11821-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using ANKRD2 Polyclonal antibody (11821-1-AP)

    IHC staining of human lung using 11821-1-AP (same clone as 11821-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung using 11821-1-AP (ANKRD2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 11821-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using ANKRD2 Polyclonal antibody (11821-1-AP)

    IHC staining of human skeletal muscle using 11821-1-AP (same clone as 11821-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 11821-1-AP (ANKRD2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 11821-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using ANKRD2 Polyclonal antibody (11821-1-AP)

    IHC staining of human skeletal muscle using 11821-1-AP (same clone as 11821-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 11821-1-AP (ANKRD2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 11821-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ANKRD2 Polyclonal antibody (11821-1-AP)

    IF Staining of HeLa using 11821-1-AP (same clone as 11821-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 11821-1-AP (ANKRD2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11821-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11821-1-PBS targets ANKRD2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ANKRD2 fusion protein Ag2389 相同性解析による交差性が予測される生物種
Full Name ankyrin repeat domain 2 (stretch responsive muscle)
Calculated molecular weight 37 aa, 2 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC020817
Gene symbol ANKRD2
Gene ID (NCBI) 26287
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ANKRD2 belongs to the conserved muscle ankyrin repeat protein (MARP) family. Expression of MARPs is induced in response to physiologic stress, injury, and hypertrophy. Together with Ankrd1/CARP and DARP, ANKRD2 are members of the MARP mechanosensing proteins that form a complex with titin (N2A)/calpain 3 protease/myopalladin. Ankrd2 is thought to have dual, structural and signaling roles, and could link the elastic I-band region as a stress sensor for transcriptional control in the nucleus