Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of mouse skeletal muscle using 11821-1-AP (same clone as 11821-1-PBS)
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11821-1-AP (ANKRD2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11821-1-PBS in a different storage buffer formulation.
WB analysis of human skeletal muscle using 11821-1-AP (same clone as 11821-1-PBS)
human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11821-1-AP (ANKRD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11821-1-PBS in a different storage buffer formulation.
IP experiment of mouse skeletal muscle using 11821-1-AP (same clone as 11821-1-PBS)
IP result of anti-ANKRD2 (IP:11821-1-AP, 4ug; Detection:11821-1-AP 1:500) with mouse skeletal muscle tissue lysate 3600ug. This data was developed using the same antibody clone with 11821-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 11821-1-AP (same clone as 11821-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 11821-1-AP (ANKRD2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 11821-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 11821-1-AP (same clone as 11821-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 11821-1-AP (ANKRD2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 11821-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 11821-1-AP (same clone as 11821-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 11821-1-AP (ANKRD2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 11821-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 11821-1-AP (same clone as 11821-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 11821-1-AP (ANKRD2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 11821-1-PBS in a different storage buffer formulation.
IHC staining of human skin using 11821-1-AP (same clone as 11821-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skin using 11821-1-AP (ANKRD2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 11821-1-PBS in a different storage buffer formulation.
IHC staining of human skin using 11821-1-AP (same clone as 11821-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skin using 11821-1-AP (ANKRD2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 11821-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 11821-1-AP (same clone as 11821-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain using 11821-1-AP (ANKRD2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 11821-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 11821-1-AP (same clone as 11821-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain using 11821-1-AP (ANKRD2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 11821-1-PBS in a different storage buffer formulation.
IHC staining of human lung using 11821-1-AP (same clone as 11821-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung using 11821-1-AP (ANKRD2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 11821-1-PBS in a different storage buffer formulation.
IHC staining of human lung using 11821-1-AP (same clone as 11821-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung using 11821-1-AP (ANKRD2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 11821-1-PBS in a different storage buffer formulation.
IHC staining of human skeletal muscle using 11821-1-AP (same clone as 11821-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 11821-1-AP (ANKRD2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 11821-1-PBS in a different storage buffer formulation.
IHC staining of human skeletal muscle using 11821-1-AP (same clone as 11821-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 11821-1-AP (ANKRD2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 11821-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 11821-1-AP (same clone as 11821-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ANKRD2 antibody (11821-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 11821-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

ANKRD2 Polyclonal antibody, PBS Only

ANKRD2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 11821-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

hArpp, ARPP, Ankyrin repeat domain-containing protein 2

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11821-1-PBS targets ANKRD2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ANKRD2 fusion protein Ag2389 相同性解析による交差性が予測される生物種
Full Name	ankyrin repeat domain 2 (stretch responsive muscle)
Calculated molecular weight	37 aa, 2 kDa
Observed molecular weight	42 kDa
GenBank accession number	BC020817
Gene Symbol	ANKRD2
Gene ID (NCBI)	26287
RRID	AB_2057858
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9GZV1
Storage Buffer	PBS only , pH 7.3
Storage Conditions	Store at -80°C.

Background Information

ANKRD2 belongs to the conserved muscle ankyrin repeat protein (MARP) family. Expression of MARPs is induced in response to physiologic stress, injury, and hypertrophy. Together with Ankrd1/CARP and DARP, ANKRD2 are members of the MARP mechanosensing proteins that form a complex with titin (N2A)/calpain 3 protease/myopalladin. Ankrd2 is thought to have dual, structural and signaling roles, and could link the elastic I-band region as a stress sensor for transcriptional control in the nucleus

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse skeletal muscle using 11821-1-AP (same clone as 11821-1-PBS)

WB analysis of human skeletal muscle using 11821-1-AP (same clone as 11821-1-PBS)

IP experiment of mouse skeletal muscle using 11821-1-AP (same clone as 11821-1-PBS)

IHC staining of human kidney using 11821-1-AP (same clone as 11821-1-PBS)

IHC staining of human kidney using 11821-1-AP (same clone as 11821-1-PBS)

IHC staining of human testis using 11821-1-AP (same clone as 11821-1-PBS)