Validation Data Gallery
Tested Applications
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SW480 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24035-1-AP targets ANKRD22 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag21248 Product name: Recombinant human ANKRD22 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 1-191 aa of BC021671 Sequence: MGILYSEPICQAAYQNDFGQVWRWVKEDSSYANVQDGFNGDTPLICACRRGHVRIVSFLLRRNANVNLKNQKERTCLHHAVKKKFTFIDYLLIILLMPVLLIGYFLMVSKTKQNEALVRMLLDAGVEVNATDCYGCTALHYACEMKNQSLIPLLLEARADPTIKNKHGESSLDIARRLKFSQIELMLRKAL 相同性解析による交差性が予測される生物種 |
| Full Name | ankyrin repeat domain 22 |
| Calculated molecular weight | 191 aa, 22 kDa |
| GenBank accession number | BC021671 |
| Gene Symbol | ANKRD22 |
| Gene ID (NCBI) | 118932 |
| RRID | AB_3669429 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | Q5VYY1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Ankyrin repeat domain-containing protein 22 (ANKRD22) is a novel N-myristoylated protein with a predicted single-pass transmembrane domain. ANKRD22 could potentially act as a novel therapeutic target for non-small cell lung cancer (NSCLC) (PMID: 28667340).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ANKRD22 antibody 24035-1-AP | Download protocol |
| IHC protocol for ANKRD22 antibody 24035-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |