Validation Data Gallery
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Tested Applications
| Positive WB detected in | MCF-7 cells |
| Positive IP detected in | A431 cells, MCF-7 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IF | See 1 publications below |
Product Information
24034-1-AP targets ANKRD27 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ANKRD27 fusion protein Ag21239 相同性解析による交差性が予測される生物種 |
| Full Name | ankyrin repeat domain 27 (VPS9 domain) |
| Calculated molecular weight | 1050 aa, 117 kDa |
| Observed molecular weight | 117-130 kDa |
| GenBank accession number | BC050529 |
| Gene Symbol | ANKRD27 |
| Gene ID (NCBI) | 84079 |
| RRID | AB_2879413 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96NW4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
VARP (VPS9-ankyrin-repeat protein, also known as ANKRD27) was originally identified as an N-terminal VPS9 (vacuolar protein sorting 9)-domain-containing protein that possesses guanine nucleotide exchange factor (GEF) activity toward small GTPase Rab21 and contains two ankyrin repeat (ANKR) domains in its central region.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ANKRD27 antibody 24034-1-AP | Download protocol |
| IHC protocol for ANKRD27 antibody 24034-1-AP | Download protocol |
| IF protocol for ANKRD27 antibody 24034-1-AP | Download protocol |
| IP protocol for ANKRD27 antibody 24034-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
EMBO J Control of RAB7 activity and localization through the retromer-TBC1D5 complex enables RAB7-dependent mitophagy.
| ||
J Cell Sci TI-VAMP/VAMP7-SNARE-Rab-GTPase interaction network within a ciliary membrane targeting complex. | ||
Autophagy RAB21 controls autophagy and cellular energy homeostasis by regulating retromer-mediated recycling of SLC2A1/GLUT1 | ||
J Cell Sci Rabin8 phosphorylated by NDR2/STK38L, the canine early retinal degeneration (erd) gene product, directs rhodopsin Golgi-to-cilia trafficking |