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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ANKRD33 Polyclonal antibody

ANKRD33 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25202-1-AP

Synonyms

ANKRD33, ankyrin repeat domain 33, C12orf7

Validation Data Gallery

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  • Western Blot (WB) analysis of Y79 cells using ANKRD33 Polyclonal antibody (25202-1-AP)

    WB analysis of Y79 using 25202-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 25202-1-AP (ANKRD33 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using ANKRD33 Polyclonal antibody (25202-1-AP)

    IHC staining of human testis using 25202-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25202-1-AP (ANKRD33 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using ANKRD33 Polyclonal antibody (25202-1-AP)

    IHC staining of human testis using 25202-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25202-1-AP (ANKRD33 Antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

Positive WB detected inY79 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25202-1-AP is ANKRD33 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ANKRD33 fusion protein Ag18582 相同性解析による交差性が予測される生物種
Full Name ankyrin repeat domain 33
Calculated molecular weight 452 aa, 49 kDa
Observed molecular weight 49 kDa
GenBank accession numberBC121153
Gene symbol ANKRD33
Gene ID (NCBI) 341405
RRIDAB_2879956
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ankyrin repeat domain 33 (ANKRD33), also named as C12orf7, belongs to the ankyrin repeat family and is highly expressed in retina. ANKRD33 exists as two isoforms. The isoform 1 molecular weight is 29kDa and isoform 2 is 49kDa. The function of ANKRD33 is still non-known.

Protocols

Product Specific Protocols
WB protocol for ANKRD33 antibody 25202-1-APDownload protocol
IHC protocol for ANKRD33 antibody 25202-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols