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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ANKRD46 Polyclonal antibody

ANKRD46 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 27568-1-AP

Synonyms

Ankyrin repeat domain-containing protein 46, ankyrin repeat domain 46, ANK-S

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ANKRD46 Polyclonal antibody (27568-1-AP)

    WB analysis using 27568-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27568-1-AP (ANKRD46 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using ANKRD46 Polyclonal antibody (27568-1-AP)

    IF Staining of HEK-293 using 27568-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ANKRD46 antibody (27568-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27568-1-AP targets ANKRD46 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ANKRD46 fusion protein Ag26623 相同性解析による交差性が予測される生物種
Full Name ankyrin repeat domain 46
Observed molecular weight 25 kDa
GenBank accession numberBC060843
Gene symbol ANKRD46
Gene ID (NCBI) 157567
RRIDAB_3669610
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ANKRD46 encodes a protein containing multiple ankyrin repeats. Ankyrin domains function in protein-protein interactions in a variety of cellular processes. ANKRD46 also known as ankyrin repeat small protein (ANK-S), is a 228-amino acid single-pass membrane protein(PMID: 21219636).

Protocols

Product Specific Protocols
WB protocol for ANKRD46 antibody 27568-1-APDownload protocol
IF protocol for ANKRD46 antibody 27568-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols