• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ANKRD50 Polyclonal antibody

ANKRD50 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 24032-1-AP

Synonyms

ANKRD50, ankyrin repeat domain 50, KIAA1223

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HEK-293 cells using ANKRD50 Polyclonal antibody (24032-1-AP)

    WB analysis of HEK-293 using 24032-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24032-1-AP (ANKRD50 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using ANKRD50 Polyclonal antibody (24032-1-AP)

    IP experiment of HEK-293 using 24032-1-AP

    IP result of anti-ANKRD50 (IP:24032-1-AP, 5ug; Detection:24032-1-AP 1:300) with HEK-293 cells lysate 4000ug.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using ANKRD50 Polyclonal antibody (24032-1-AP)

    IF Staining of HEK-293 using 24032-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 24032-1-AP (ANKRD50 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Positive WB detected inHEK-293 cells
Positive IP detected inHEK-293 cells
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24032-1-AP is ANKRD50 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ANKRD50 fusion protein Ag21237 相同性解析による交差性が予測される生物種
Full Name ankyrin repeat domain 50
Calculated molecular weight 1429 aa, 156 kDa
Observed molecular weight 137-156 kDa
GenBank accession numberBC024725
Gene symbol ANKRD50
Gene ID (NCBI) 57182
RRIDAB_2879411
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ANKRD50 antibody 24032-1-APDownload protocol
IF protocol for ANKRD50 antibody 24032-1-APDownload protocol
IP protocol for ANKRD50 antibody 24032-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols