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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ANKS1B Polyclonal antibody

ANKS1B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 24783-1-AP

Synonyms

AIDA, AIDA 1, ANKS1B, ANKS2, cajalin 2, E2A PBX1 associated protein, EB 1, EB1

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using ANKS1B Polyclonal antibody (24783-1-AP)

    WB analysis of A549 using 24783-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 24783-1-AP (ANKS1B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using ANKS1B Polyclonal antibody (24783-1-AP)

    WB analysis of mouse testis using 24783-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 24783-1-AP (ANKS1B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ANKS1B Polyclonal antibody (24783-1-AP)

    WB analysis of HeLa using 24783-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24783-1-AP (ANKS1B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using ANKS1B Polyclonal antibody (24783-1-AP)

    IP experiment of HeLa using 24783-1-AP

    IP result of anti-ANKS1B (IP:24783-1-AP, 4ug; Detection:24783-1-AP 1:1000) with HeLa cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ANKS1B Polyclonal antibody (24783-1-AP)

    IHC staining of mouse brain using 24783-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24783-1-AP (ANKS1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ANKS1B Polyclonal antibody (24783-1-AP)

    IHC staining of mouse brain using 24783-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24783-1-AP (ANKS1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ANKS1B Polyclonal antibody (24783-1-AP)

    IF Staining of HepG2 using 24783-1-AP

    Immunofluorescent analysis of HepG2 cells using 24783-1-AP (ANKS1B antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

Positive WB detected inA549 cells, HeLa cells, mouse testis tissue
Positive IP detected inHeLa cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 24783-1-AP is ANKS1B Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ANKS1B fusion protein Ag20454 相同性解析による交差性が予測される生物種
Full Name ankyrin repeat and sterile alpha motif domain containing 1B
Calculated molecular weight 1248 aa, 138 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC026313
Gene symbol ANKS1B
Gene ID (NCBI) 56899
RRIDAB_2879720
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ANKS1B also named as AIDA or cajalin 2 is a amino acid protein, which contains 7 ANK repeats, 1 PID domain and 2 SAM domains. ANKS1B localizes in cytoplasm and is highly expressed in brain and testis. ANKS1B exists as many alternatively spliced isoforms. It interacts with AbPP to play a role in brain development. AIDA-1 also interacts with coilin in Cajal bodies to regulate pre-mRNA splicing.

Protocols

Product Specific Protocols
WB protocol for ANKS1B antibody 24783-1-APDownload protocol
IHC protocol for ANKS1B antibody 24783-1-APDownload protocol
IF protocol for ANKS1B antibody 24783-1-APDownload protocol
IP protocol for ANKS1B antibody 24783-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EBioMedicine

Juvenile CLN3 disease is a lysosomal cholesterol storage disorder: similarities with Niemann-Pick type C disease

Authors - Jacinda Chen
View Article