ANKZF1 Polyclonal antibody

Name: ANKZF1 Polyclonal antibody
Brand: Proteintech
SKU: 20447-1-AP

ANKZF1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 20447-1-AP

Synonyms

ANKZF1, Zinc finger protein 744, ZNF744

Validation Data Gallery

Filter:

AllWBIHC

WB analysis of BxPC-3 using 20447-1-AP
BxPC-3 cells were subjected to SDS PAGE followed by western blot with 20447-1-AP (ANKZF1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 20447-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 20447-1-AP (ANKZF1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 20447-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 20447-1-AP (ANKZF1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 20447-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 20447-1-AP (ANKZF1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 20447-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 20447-1-AP (ANKZF1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 20447-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 20447-1-AP (ANKZF1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 20447-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 20447-1-AP (ANKZF1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human liver using 20447-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20447-1-AP (ANKZF1 antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human liver using 20447-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20447-1-AP (ANKZF1 antibody) at dilution of 1:200 (under 10x lens).

-/ -

Cat No. 20447-1-AP

Size/Concentration

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	BxPC-3 cells, COLO 320 cells, HeLa cells, HepG2 cells, MCF-7 cells
Positive IHC detected in	human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 5 publications below
IHC	See 1 publications below
IF	See 1 publications below

Product Information

The immunogen of 20447-1-AP is ANKZF1 Fusion Protein expressed in E. coli.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ANKZF1 fusion protein Ag14285 相同性解析による交差性が予測される生物種
Full Name	ankyrin repeat and zinc finger domain containing 1
Calculated molecular weight	726 aa, 81 kDa
Observed molecular weight	81 kDa
GenBank accession number	BC000238
Gene symbol	ANKZF1
Gene ID (NCBI)	55139
RRID	AB_2878688
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for ANKZF1 antibody 20447-1-AP	Download protocol
IHC protocol for ANKZF1 antibody 20447-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Commun Stalled translation by mitochondrial stress upregulates a CNOT4-ZNF598 ribosomal quality control pathway important for tissue homeostasis Authors - Ji Geng View Article
human	WB,IF	Acta Neuropathol Commun Inefficient quality control of ribosome stalling during APP synthesis generates CAT-tailed species that precipitate hallmarks of Alzheimer's disease Authors - Suman Rimal View Article
human	WB	bioRxiv Ribosomal quality control factors inhibit repeat-associated non-AUG translation from GC-rich repeats Authors - Yi-Ju Tseng View Article
human	WB	Nucleic Acids Res Ribosomal quality control factors inhibit repeat-associated non-AUG translation from GC-rich repeats Authors - Yi-Ju Tseng View Article
human	WB,IHC	Oncol Rep Overexpression of ZNF169 promotes the growth and proliferation of colorectal cancer cells via the upregulation of ANKZF1 Authors - Jie Zhang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ANKZF1 Polyclonal antibody

ANKZF1 Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis of BxPC-3 using 20447-1-AP

WB analysis of MCF-7 using 20447-1-AP

WB analysis of HeLa using 20447-1-AP

WB analysis of HepG2 using 20447-1-AP

WB analysis of HeLa using 20447-1-AP

WB analysis of COLO 320 using 20447-1-AP

WB analysis of MCF-7 using 20447-1-AP

IHC staining of human liver using 20447-1-AP

IHC staining of human liver using 20447-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Stalled translation by mitochondrial stress upregulates a CNOT4-ZNF598 ribosomal quality control pathway important for tissue homeostasis

Inefficient quality control of ribosome stalling during APP synthesis generates CAT-tailed species that precipitate hallmarks of Alzheimer's disease

Ribosomal quality control factors inhibit repeat-associated non-AUG translation from GC-rich repeats

Ribosomal quality control factors inhibit repeat-associated non-AUG translation from GC-rich repeats

Overexpression of ZNF169 promotes the growth and proliferation of colorectal cancer cells via the upregulation of ANKZF1