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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ANLN Monoclonal antibody (66643-1-Ig)

    WB analysis using 66643-1-Ig (same clone as 66643-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66643-1-Ig (ANLN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66643-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using ANLN Monoclonal antibody (66643-1-Ig)

    WB analysis of MCF-7 using 66643-1-Ig (same clone as 66643-1-PBS)

    WB result of ANLN antibody (66643-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ANLN transfected MCF-7 cells. This data was developed using the same antibody clone with 66643-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using ANLN Monoclonal antibody (66643-1-Ig)

    WB analysis of rat brain using 66643-1-Ig (same clone as 66643-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66643-1-Ig (ANLN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66643-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using ANLN Monoclonal antibody (66643-1-Ig)

    WB analysis of mouse brain using 66643-1-Ig (same clone as 66643-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 66643-1-Ig (ANLN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66643-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using ANLN Monoclonal antibody (66643-1-Ig)

    IF Staining of A431 using 66643-1-Ig (same clone as 66643-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using ANLN antibody (66643-1-Ig, Clone: 1B4A8 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66643-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66643-1-PBS targets ANLN in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ANLN fusion protein Ag19294 相同性解析による交差性が予測される生物種
Full Name anillin, actin binding protein
Calculated molecular weight 1124 aa, 124 kDa
Observed molecular weight 110 kDa~120 kDa
GenBank accession numberBC034692
Gene Symbol ANLN
Gene ID (NCBI) 54443
RRIDAB_2815000
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9NQW6
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Anillin, an actin-binding protein, is a conserved cleavage furrow component important for cytokinesis. The human anillin protein localizes to the nucleus during interphase, the cortex following nuclear envelope breakdown, and the cleavage furrow during cytokinesis. It interactions with at least two other furrow proteins, actin and the septins through its C-terminal PH domain and N-terminal actin-binding domain. In anillin knockdown cells, the cleavage furrow ingressed but failed to complete the ingression. Anillin has been found to be overexpressed in tumors and may serve as promising marker for pancreatic carcinoma molecular diagnostics as well as a prognostic indicator for non-small cell lung cancers.