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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ANO10 Polyclonal antibody

ANO10 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21901-1-AP

Synonyms

ANO10, anoctamin 10, TMEM16K, Transmembrane protein 16K

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using ANO10 Polyclonal antibody (21901-1-AP)

    WB analysis of mouse brain using 21901-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 21901-1-AP (ANO10 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of mouse brain tissue using ANO10 Polyclonal antibody (21901-1-AP)

    WB analysis of mouse brain using 21901-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 21901-1-AP (ANO10 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using ANO10 Polyclonal antibody (21901-1-AP)

    IP experiment of mouse brain using 21901-1-AP

    IP result of anti-ANO10 (IP:21901-1-AP, 3ug; Detection:21901-1-AP 1:500) with mouse brain tissue lysate 3600ug.

  • Immunohistochemistry (IHC) staining of human testis tissue using ANO10 Polyclonal antibody (21901-1-AP)

    IHC staining of human testis using 21901-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 21901-1-AP (ANO10 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using ANO10 Polyclonal antibody (21901-1-AP)

    IHC staining of human testis using 21901-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 21901-1-AP (ANO10 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

Positive WB detected inmouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

The immunogen of 21901-1-AP is ANO10 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ANO10 fusion protein Ag13983 相同性解析による交差性が予測される生物種
Full Name anoctamin 10
Calculated molecular weight 660 aa, 76 kDa
Observed molecular weight 76 kDa
GenBank accession numberBC038855
Gene symbol ANO10
Gene ID (NCBI) 55129
RRIDAB_2878940
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ANO10, also named as TMEM16K, is 660 amino acid protein, which belongs to the anoctamin family. ANO10 does not exhibit calcium-activated chloride channel activity and can inhibit the activity of ANO1. ANO10 is Highly expressed in the brain. ANO10 may have an association with Spinocerebellar ataxia, autosomal recessive, 10.

Protocols

Product Specific Protocols
WB protocol for ANO10 antibody 21901-1-APDownload protocol
IHC protocol for ANO10 antibody 21901-1-APDownload protocol
IP protocol for ANO10 antibody 21901-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Am J Cancer Res

ANO10 is a potential prognostic biomarker and correlates with immune infiltration in breast cancer

Authors - Ran Ning
View Article