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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ANO3 Polyclonal antibody

ANO3 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF, IP, ELISA

Conjugate

Unconjugated

Cat no : 19489-1-AP

Synonyms

Transmembrane protein 16C, TMEM16C, GENX-3947, C11orf25, Anoctamin-3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ANO3 Polyclonal antibody (19489-1-AP)

    WB analysis using 19489-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19489-1-AP (ANO3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skin tissue using ANO3 Polyclonal antibody (19489-1-AP)

    WB analysis of mouse skin using 19489-1-AP

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 19489-1-AP (ANO3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse ovary tissue using ANO3 Polyclonal antibody (19489-1-AP)

    WB analysis of mouse ovary using 19489-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 19489-1-AP (ANO3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using ANO3 Polyclonal antibody (19489-1-AP)

    IP experiment of mouse testis using 19489-1-AP

    IP result of anti-ANO3 (IP:19489-1-AP, 4ug; Detection:19489-1-AP 1:1000) with mouse testis tissue lysate 4000ug.

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Tested Applications

Positive WB detected inmouse brain tissue, mouse skin tissue, mouse ovary tissue, mouse kidney tissue
Positive IP detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19489-1-AP targets ANO3 in WB, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityrat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name anoctamin 3
Calculated molecular weight 115 kDa
Observed molecular weight 100-115 kDa
GenBank accession numberNM_031418
Gene symbol ANO3
Gene ID (NCBI) 63982
RRIDAB_10638489
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ANO3, also named as C11orf25, TMEM16C and GENX-3947, belongs to the anoctamin family. It may act as a calcium-activated chloride channel.

Protocols

Product Specific Protocols
WB protocol for ANO3 antibody 19489-1-APDownload protocol
IP protocol for ANO3 antibody 19489-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIP, IF

Neurosci Bull

A Role for Transmembrane Protein 16C/Slack Impairment in Excitatory Nociceptive Synaptic Plasticity in the Pathogenesis of Remifentanil-induced Hyperalgesia in Rats.

Authors - Yize Li
View Article